Mapping a high oleic acid mutation in winter oilseed rape (Brassica napus L.) |
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| Authors: | A Schierholt H C Becker W Ecke |
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| Institution: | Institute of Agronomy and Plant Breeding, University of G?ttingen; Von Siebold Strasse 8, 37075 G?ttingen; Germany e-mail: aschier@gwdg.de, DE
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| Abstract: | Two winter oilseed rape mutant lines, 7488 and 19661, with a high oleic (HO) acid content in the seed oil were characterized
phenotypically. In both mutant lines the HO trait was monogenically inherited. Segregation analysis in an F2 population derived from a cross between 7488 and 19661 showed the two mutations to be allelic. From a comparison of seed,
leaf and root fatty acid composition it was concluded that fad2, the endoplasmic oleic acid desaturase, is affected by the mutation. In a bulked segregant analysis three AFLP markers linked
to this mutation were detected and localized on the genetic map of Brassica napus. The markers mapped near the locus of one copy of the fad2 gene in the rapeseed genome.
Received: 16 February 2000 / Accepted: 28 March 2000 |
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| Keywords: | Brassica napus L Molecular marker AFLP fad2 High oleic acid Fatty acids Plant lipids |
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