| 乙型肝炎病毒e抗原基因与绿色荧光蛋白基因的融合及其表达 |
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| 引用本文： | 岳莉莉,邓小昭.乙型肝炎病毒e抗原基因与绿色荧光蛋白基因的融合及其表达[J].Virologica Sinica,1997,12(4):336-341. |
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| 作者姓名： | 岳莉莉 邓小昭 |
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| 作者单位： | 武汉大学病毒研究所!武汉，430072，武汉大学病毒研究所!武汉，430072，武汉大学病毒研究所!武汉，430072，武汉大学病毒研究所!武汉，430072，南京军区军事医学研究所!南京，210002 |
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| 摘 要： | 质粒pS65T含有T7启动子驱动的绿色荧光蛋白突变型gfpS65T基因，经修饰后，在其中插入乙型肝炎病毒e抗原（HBeAg）基因，使两基因同框成为融合基因。在大肠杆菌BL21中由于T7启动子的控制，高效表达了具有双功能（抗原性和发光性）的融合蛋白（GFP－HBeAg）。融合蛋白MW为52kDa，N端有6个组氨酸残基，因而用Ni金属螯合层析柱对融合蛋白进行了分离纯化，利用诊断HBV的ELISA试剂盒检测了融合蛋白的抗原性，在荧光显微镜下观察到了融合蛋白的绿色荧光。并探讨了用其组装成新型免疫诊断试剂的可能性。
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| 关 键 词： | 乙型肝炎病毒e抗原基因 绿色荧光蛋白基因 T7噬菌体启动子 双功能融合蛋白 |
Fusion of HBV e Gene with Green Fluorescent Protein Gene and Its Expression in E. coli Cells |
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| Deng Xiaozhao.Fusion of HBV e Gene with Green Fluorescent Protein Gene and Its Expression in E. coli Cells[J].中国病毒学(英文版),1997,12(4):336-341. |
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| Authors: | Deng Xiaozhao |
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| Institution: | (Yue Lili, Qi Yipeng, Deng Yanhui, Lu Liqun)(Institute of Virology,Wuhan University,Wuhan 430072)Deng Xiaozhao (Nanjing Institute of Military Medical Sciences,Nanjing 210002) |
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| Abstract: | The bi - function fusion protein of hepatitis B virus e antigen and mutant green fluorescent protein was expressed in E. coli BL21 strain. The fusion protein was separated and its anti-genecity was determined by ELISA kit for HBe, meanwhile the intensity of its luminescence could be observed. The approach in which the antigen was tagged by equal molecular GFP allows using the bi-function fusion protein to establish a new method for immunogical diagnosis. |
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| Keywords: | HBeAg gene Green fluorescent protein gene T7 phage promotor Bi-function protein |
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