核型多角体病毒的增值与棉铃虫血淋巴酯酶的变化

核型多角体病毒（NPV）对同源棉铃虫有很强的毒杀作用，是重要杀虫资源。病毒感染导致宿主代谢紊乱，引起一系列生化反应的变化，可通过一定方法观察到其表征，此为病毒感染的病理生化特征。昆虫血淋巴对于病毒感染引起的全身症状具有重要作用，并影响宿主物质和能量代谢的性质与水平，反映出病毒一宿主的互作关系。本文以血淋巴酯酶同工酶（EST）为感病指标，对其感染变化进行研究。1材料和方法11材料11．呈供试病毒棉铃虫核型多用体病毒．取自湖北仙桃磷肥厂生化分厂。1．1．2供试昆虫棉铃虫三龄幼虫，由该厂提供，半人工饲料饲养。豆…

The Multiplication of Nuclear Polyhedrosis Viruses and Changes of Hemolymph Esterases in Helicoverpa armigera Larvaes after Infection with NPV

By the polyacrylamide gel electrophoresis (PAGE) and thin-layer scanning, the esterases (EST) of the third instar larvae of the Helicoverpa armigera were investigated in qualityand quantity after infection with nuclear polyhedrosis viruses (NPV). The changes in sort andcontent of hemolymph EST were examined at six hours postinfection and the numbers of ESTbands were from more to less as develop and infective time in larvae. The varies in EST patternbetween infection and control were not significant but the content of each band and the accumulation of EST were obvious. According to the changeable regularity in EST content of zymogram,the total and single content in diseased larvaes always gradually decreased with passages of infective time but in the healthy larvaes had no distinct changes. The results demonstrated that the infection with NPV could affect the EST metabolism in larvae hemolymph, and the metabolicchanges may show the infective level and extent, there fore it had indicated function.