| PCR检测HPV－18DNA |
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| 引用本文： | 江红,柯丽华.PCR检测HPV－18DNA[J].Virologica Sinica,1994(1). |
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| 作者姓名： | 江红 柯丽华 |
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| 作者单位： | 中国科学院武汉病毒研究所 |
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| 摘 要： | 采用聚合酶链式反应（ＰＣＲ）技术，对ＨＰＶ－１８序列中引物ＨＰ＿１、ＨＰ＿２之间的片段（Ｆ）进行扩增，通过两组阴、阳性对照实验证明扩增片段的特异性。用不同Ｍｇ浓度的缓冲系统进行ＰＣＲ反应发现，缓冲系统中Ｍｇ浓度高低是影响ＨＰＶ－１８／ＨＰ＿１、ＨＰ＿２特异扩增的重要因素，高浓度Ｍｇ导致扩增特异性降低。对１７例宫颈癌组织ＤＮＡ进行ＰＣＲ检测，有９例检出Ｆ片段，其检出率是５３％，为ＨＰＶ－１８与宫颈癌的相关性提供证据。
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| 关 键 词： | 人乳头瘤病毒18型（HPV－18），聚合酶链式反应（PCR），宫颈癌，特异性，检测 |
Detection of HPV-18 DNA With PCR |
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| Jiang Hong,Ke Lihua.Detection of HPV-18 DNA With PCR[J].中国病毒学(英文版),1994(1). |
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| Authors: | Jiang Hong Ke Lihua |
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| Abstract: | he DNA fragment(F)on the Upetream Regulatory Region(URR)of HPV-18 DNA was amplified with PCR.Two control experiments here confirmed the PCR specificity.The coneentration ofMg in PCR buffer affected mostly the specificity of PCR,the higher the coneentration of Mg,thelower the specificity,Moreover,nine out of seventeen(53%)DNA samples of cervical carcinoma tissues were detected with PcR for the presence of HVP-18 URR F sequences.It's implied that the re-sult may be an evidence of the relationship between HPV-18 and cervical carcinoma. |
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| Keywords: | HPV-18 PCR Cervical carcinoma Specificity Detection |
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