| 生长素结合蛋白cDNA的克隆及其在黄瓜中的表达 |
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| 引用本文： | 白吉刚,宋明,刘佩瑛,张盛林,王中凤.生长素结合蛋白cDNA的克隆及其在黄瓜中的表达[J].植物学通报,2002,19(6):705-709. |
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| 作者姓名： | 白吉刚 宋明 刘佩瑛 张盛林 王中凤 |
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| 作者单位： | 西南农业大学园艺系,重庆,400716 |
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| 基金项目： | 重庆市科委应用基础项目 (2 0 0 1 1 1 2 5 ) |
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| 摘 要： | 利用RT_PCR方法从拟南芥 (Arabidopsisthaliana (L .)Heynh .)中克隆了生长素结合蛋白 (auxinbindingprotein 1 )cDNA ,进行了全序列测定。将该基因克隆在pBI1 2 1的 35S启动子和Nos终止子之间 ,得到植物表达载体p35EZ。通过根癌农杆菌 (Agrobacteriumtumefaciens (SmithetTownsend)Conn)介导的方法转化黄瓜 (CucumissativusL .)。对转基因植株进行了PCR和Southern检测。所得到的转基因黄瓜植株单性结实能力增强。
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| 关 键 词： | 拟南芥 生长素结合蛋白 黄瓜 转基因 |
| 修稿时间： | 2001年12月21 |
Cloning of Auxin-binding Protein 1 Cdna and Its Expression in Cucumber |
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| Abstract: | An auxin_binding protein 1 cDNA was isolated from Arabidopsis thaliana (L.) Heynh.by RT_PCR and confirmed by DNA sequencing. Plant expression vector p35EZ was constructed to transform the explants of Cucumis sativus L. by Agrobacterium tumefaciens (Smith et Townsend) Conn Ti plasmid_mediated method. The intergration of the transgene was identified by PCR and Southern blot analysis. It was found that the parthenocarpic ability of transgenic cucumber plants was enhanced. |
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| Keywords: | Arabidopsis Auxin_binding protein 1 Cucumis sativus Transgene |
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