| RFLP、RAPD、AFLP在水稻农垦58S和1514中多态性比较 |
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| 引用本文： | 陈亮,梅明华,徐才国,李子银,陈睦传,沈明山.RFLP、RAPD、AFLP在水稻农垦58S和1514中多态性比较[J].植物学通报,2000,17(5):424-428. |
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| 作者姓名： | 陈亮 梅明华 徐才国 李子银 陈睦传 沈明山 |
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| 作者单位： | [1]厦门大学细胞生物学与肿瘤细胞工程教育部重点实验室,厦门 [2]华中农业大学作物遗传改良国？,厦门 |
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| 摘 要： | 本文用ＲＦＬＰ、ＲＡＰＤ和ＡＦＬＰ三种分子标记技术对农垦５８ＳＸ１５１４组合及其Ｆ２极性集团进行了分析，比较了它们多成性和阳性的比率，结果显示，三种分子标记的多态性和与目的基因连锁的阳性比率分别为１９．９３％，５．２３％；１１．１７％，０．７６％和８６．４７％，７．５２％。ＡＦＬＰ的多态性比率和阳性比率均为最高。分析探讨了三种分子标记技术的优缺点及其在区间高分辩率作图和筛选与目的基因连锁标记中的运
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| 关 键 词： | RFLP RAPD AFLP 水稻 多态性比率 阳性标记比率 |
| 修稿时间： | 2000-05-15 |
Comparison
of Polymorphism of RFLP, RAPD and AFLP
in Rice Nongken 58S and 1514 |
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| CHEN Liang,MEI Ming_Hua,XU Cai_Guo,Li Zi_Yin,CHEN Mu_Zhuan,SHEN Ming_Shan WTBZ.Comparison
of Polymorphism of RFLP, RAPD and AFLP
in Rice Nongken 58S and 1514[J].Chinese Bulletin of Botany,2000,17(5):424-428. |
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| Authors: | CHEN Liang MEI Ming_Hua XU Cai_Guo Li Zi_Yin CHEN Mu_Zhuan SHEN Ming_Shan [WTBZ] |
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| Institution: | CHEN Liang,2MEI Ming_Hua,2XU Cai_Guo,3Li Zi_Yin,1CHEN Mu_Zhuan,1SHEN Ming_Shan [WT6BZ] 1 |
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| Abstract: | RFLP, RAPD and AFLP analysis were performed between Nongken 58S and 1514, two varieties of rice, and their bulked extremes of F 2. The rate of polymorphism and positive marker linked to target gene was compared. The results indicated that the rates of polymorphism and positive marker of RFLP, RAPD and AFLP are 19.93%, 5.23%;11.17%, 0.76% and 86.47%, 7.52%, respectively. The rate of polymorphism and positive marker of AFLP is highest in the three kinds of molecular marker systems. It was discussed that the advantages and disadvantages, and application of the three kinds of molecular marker systems in constructing a high resolution map of a specific chromosome region and selecting linkage markers to target gene. |
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| Keywords: | RFLP RAPD AFLP Polymorphism Rice |
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