| 大头蛙Sox基因的克隆与序列分析 |
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| 引用本文： | 陈冬生,张际峰,聂刘旺.大头蛙Sox基因的克隆与序列分析[J].生物学杂志,2005,22(3):13-15. |
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| 作者姓名： | 陈冬生 张际峰 聂刘旺 |
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| 作者单位： | 安徽师范大学生命科学学院,芜湖,241000 |
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| 基金项目： | 安徽省教育厅自然科学基金(2002kj128) |
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| 摘 要： | 参考人SRY基因HMC—box的保守区序列，设计一对特异引物，采用PCR技术扩增了大头蛙的Sox基因，并对扩增产物进行了克隆与测序。结果在雌雄个体中均扩增出217bp的基因片段，与人对照相同。序列分析表明，大头蛙雌雄个体之间Sox序列没有差异，与人SOX基因的同源性达到88％，与其它各类动物的Sox基因也都有非常高的相似性。结果支持Sox基因在进化上十分保守的结论。
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| 关 键 词： | 大头蛙 Sox基因 克隆 序列分析 |
| 文章编号： | 1008-9632(2005)03-0013-03 |
| 修稿时间： | 2004年11月8日 |
The clone and sequence analysis of the Sox gene in Rana kuhlii |
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| CHEN Dong-sheng,ZHANG Ji-Feng,NIE Liu-Wang.The clone and sequence analysis of the Sox gene in Rana kuhlii[J].Journal of Biology,2005,22(3):13-15. |
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| Authors: | CHEN Dong-sheng ZHANG Ji-Feng NIE Liu-Wang |
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| Abstract: | Using PCR,The Sox genes of Rana kuhlii were amplified with a pair of primers,based on the human SRY gene,Then the amplication products were cloned and sequenced.The results showed that both male and female frog have the Sox gene,which length were all 217bp and equal to that of the human SRY gene.The result of sequencing showed there was no differences in the Sox genes between the male and the female frogs.Sequece analysises indicated there were high identities between frog and many other animals including human being.The result of this paper supported the argument that Sox gene is highly conservative in phylogenetic. |
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| Keywords: | Rana kuhlii Sox gene clone sequence analysis |
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