| 微波-纤维素酶法提取枸杞多糖的工艺研究 |
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| 引用本文： | 王启为,晋晓勇,全晓塞,马建龙.微波-纤维素酶法提取枸杞多糖的工艺研究[J].生物学杂志,2013(6):95-98,102. |
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| 作者姓名： | 王启为 晋晓勇 全晓塞 马建龙 |
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| 作者单位： | 宁夏大学化学化工学院,银川750021 |
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| 基金项目： | 国家自然科学基金：基于生物条形码技术检测食源性致病菌的生物传感器研究（21067010）; 宁夏自然科学基金项目：纳米材料信号放大技术用于食源性致病菌检测方法研究（NZ1004） |
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| 摘 要： | 枸杞为常用的中药材,其多糖含量高。枸杞多糖在保健、医疗方面应用广泛,目前已有不少枸杞多糖提取方法的研究,但尚无微波-纤维素酶法提取枸杞多糖的研究。为了更好地提取枸杞多糖,满足日益增长的需求,进行了相关研究。最佳工艺条件为：微波功率450 W,微波处理时间6 min,物料为80目,液料比25∶1（mL/g）,纤维素酶用量1.5%,酶解温度50℃,酶解时间60 min,酶解体系pH值4.8,在此工艺条件下,枸杞多糖得率为13.2%。
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| 关 键 词： | 微波 枸杞 多糖 纤维素酶 |
The study of extraction polysaccharide from Lycium barbarum by microwave-enzyme synergistic method |
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| WANG Qi-wei;JIN Xiao-yong;QUAN Xiao-sai;MA Jian-long.The study of extraction polysaccharide from Lycium barbarum by microwave-enzyme synergistic method[J].Journal of Biology,2013(6):95-98,102. |
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| Authors: | WANG Qi-wei;JIN Xiao-yong;QUAN Xiao-sai;MA Jian-long |
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| Institution: | WANG Qi-wei;JIN Xiao-yong;QUAN Xiao-sai;MA Jian-long;School of Chemistry and Chemical Engineering,Ningxia University; |
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| Abstract: | Lycium barbarum is a commonly-used medicine with high polysaccharide content,it is widely used in the health care,medical. The extraction polysaccharides from the Lycium barbarum with a commercial cellulase were prepared under microwave assistance. The present study aimed at developing an optimal procedure for the extraction of polysaccharides. The reaction was operated at temperature 50 ℃ for 60 min under the follow conditions： microwave powder 450 W for 6 min treatment,grain size 80 mesh,water/material ratio 25： 1（ mL： g）,enzyme dosage 1. 5%,pH 4. 8. Under the optimal conditions,a maximum polysaccharides yield of up to 13. 25% was obtained. |
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| Keywords: | microwave Lycium barbarum polysaccharides cellulase |
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