灰树花深层发酵培养基的研究

灰树花具有较宽广的碳源谱和氮源谱，其最佳碳源为马铃薯汁加葡萄糖，最佳氮源为麸皮。在有生长促进剂一板栗壳煮汁作用下，菌丝的增产率达１４０％。培养基的氮源种类和生长促进剂对菌丝生长具显著的影响，两者交互作用明显。培养基中碳源浓度过高不利于菌丝的生长。灰树花深层发酵的较佳培养基为ＱＦ培养基：葡萄糖６０９，ＫＨ_２ＰＯ_４ ｌｇ，ＭｇＳＯ_４ ０．５ｇ，ＣａＣｌ_２ ０．１ｇ板栗壳煮汁 １５０ｇ，水１Ｌ。

STUDY ON MEDIUM OF SUBMERGED CULTURE FOR GRIFOLA FRONDOSA

Grifola frondosa lakes advantage of rich sources of carbon and nitrogen. The optinum carbon source and nitrogen source are potato juice with glucose and wheat bran respectively. When there was a growth promoter-juice of Chinese chestnut shell in the medium, the mycelian growth rate reach 140%. The kinds of itrogen and growth promoter influenced mycelian growth obviously, and there were obvious effects with each other, too. The concentration of carbon source was too high in the medium was unfavorable to growth. In addition, the better submerged culture medium of Grifola frondosa was QF: glucose 60g, KH_2PO_4lg, MgSO_4 0.59, CaCl_2 0.1g, juice of Chinese chestnut shell 150g, water lL.