| 层孔菌产漆酶的摇瓶最适培养条件研究 |
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| 引用本文： | 康从宝,赵建,李清心,曲音波,高培基.层孔菌产漆酶的摇瓶最适培养条件研究[J].微生物学通报,2002,29(3):42-45. |
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| 作者姓名： | 康从宝 赵建 李清心 曲音波 高培基 |
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| 作者单位： | 山东大学微生物技术国家重点实验室,济南,250100 |
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| 基金项目： | 国家自然科学基金资助项目 (No. 2 0 0 770 1 5 )，华南理工大学制浆造纸工程国家重点实验室开放基金资助项目 |
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| 摘 要： | 筛选到一株高产漆酶的层孔菌W－1，对其兴体产漆酶的培养条件进行了优化，确定了最适的碳源、氮源，并用正交试验确定了培养基中碳源和氮源的浓度。在最优化的培养条件下，培养7d后，W－1产生的漆酶活力可以达到7．1U／mL。采用补料的方法，可以得到大量的漆酶粗酶液。
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| 关 键 词： | 层孔菌 漆酶 摇瓶 最适培养条件 白腐菌 正交试验 |
| 文章编号： | 0253-2654(2002)03-0042-04 |
| 修稿时间： | 2001年3月1日 |
STUDY ON LIQUID CONDITIONS IN SHAKING FLASKS FOR LACCASE PRODUCTION BY A WHITE ROT FUNGI |
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| KANG Cong-Bao ZHAO Jian LI Qing-Xin,QU Yin-Bo GAO Pei-Ji.STUDY ON LIQUID CONDITIONS IN SHAKING FLASKS FOR LACCASE PRODUCTION BY A WHITE ROT FUNGI[J].Microbiology,2002,29(3):42-45. |
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| Authors: | KANG Cong-Bao ZHAO Jian LI Qing-Xin QU Yin-Bo GAO Pei-Ji |
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| Abstract: | A white-rot fungi Rigidoporus sp.W-1 which could produce laccase was isolated. The fermentation conditions in shaking flasks were investigated. The optimal carbon source was wheat bran and (NH 4) 2SO 4 was the optimal nitrogen source. The components of the medium were optimized by orthogonal experiment. When W-1 was cultured under the optimum conditions, the activity of laccase could get to 7.1U/mL in 7 days.A great amount of crude laccase could be obtained by adding fresh medium to the 7 days old mycelium. |
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| Keywords: | White rot fungus Laccase Orthogonal experiment |
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