| 土壤细菌DNA提取及多样性分析的T-RFLP方法 |
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| 引用本文： | 葛芸英,陈 松,胡 兰,凃 政.土壤细菌DNA提取及多样性分析的T-RFLP方法[J].微生物学通报,2008,35(1):131-136. |
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| 作者姓名： | 葛芸英 陈 松 胡 兰 凃 政 |
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| 作者单位： | 公安部物证鉴定中心,北京,100038 |
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| 基金项目： | 公安部重点研究项目（No.20038221401） |
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| 摘 要： | 获得高质量的土壤总DNA是土壤细菌生态学的关键步骤之一.实验通过综合应用两个试剂盒(Soilmaster kit和DNA IQTM系统)的优点进行土壤样品总DNA的提取,结果证明该方法是一种快速、有效、灵敏、稳定的土壤DNA提取方法.另外尝试将16S rDNA序列和T-RFLP(Terminal restriction fragment 1ength polymorphism)技术引入土壤细菌DNA群落多样性的研究中,证明T-RFLP是一种有力的土壤细菌多样性分析工具.
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| 关 键 词： | 土壤细菌 多样性分析 DNA提取 T-RFLP分析 土壤细菌 提取 多样性分析 方法 Diversity Analysis DNA Extraction Bacterial Soil 研究 群落多样性 Terminal restriction fragment polymorphism 序列 rDNA 稳定 快速 结果 |
| 收稿时间： | 2006-12-18 |
| 修稿时间： | 2007-10-19 |
A New Method of Soil Bacterial DNA Extraction and T-RFLP Analysis for Diversity |
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| GE Yun-Ying,CHEN Song,HU Lan and TU Zheng.A New Method of Soil Bacterial DNA Extraction and T-RFLP Analysis for Diversity[J].Microbiology,2008,35(1):131-136. |
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| Authors: | GE Yun-Ying CHEN Song HU Lan and TU Zheng |
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| Institution: | Institute of Forensic Science, Ministry of Public Security, Beijing 100038;Institute of Forensic Science, Ministry of Public Security, Beijing 100038;Institute of Forensic Science, Ministry of Public Security, Beijing 100038;Institute of Forensic Science, Ministry of Public Security, Beijing 100038 |
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| Abstract: | Obtaining soil bacterial DNA of good quality is a key step in soil bacterial ecology study. A quick, efficient, sensitive and stably method of DNA extraction from soil were established by combining strongpoints of two kits ( Soilmaster kit and DNA IQTM kit). In addition, the 16S rDNA gene and T-RFLP (Terminal restriction fragment length polymorphism) were used in the analysis of soil bacterial community diversity and the result show that T-RFLP is a powerful tool for bacterial community study. |
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| Keywords: | Soil bacterial Diversity analysis DNA extraction T-RFLP |
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