Molecular tagging and mapping of the erect panicle gene in rice |
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Authors: | Fan Na Kong Jia Yu Wang Ji Cheng Zou Li Xue Shi Min De Jin Zheng Jin Xu Bin Wang |
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Institution: | (1) The State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China;(2) The Graduate School of Chinese Academy of Sciences, Beijing, 100039, China;(3) College of Agronomy, Shenyang Agricultural University, Shenyang, 110161, China |
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Abstract: | Erect panicle (EP) is one of the more important traits of the proposed ideotype of high-yielding rice. Several rice cultivars
with the EP phenotype, which has been reported to be controlled by a dominant gene, have been successfully developed and released
for commercial production in North China. To analyze the inheritance of the EP trait, we generated segregating F2 and BC1F1 populations by crossing an EP-type variety, Liaojing 5, and a curved-panicle-type variety, Fengjin. Our results confirmed that a dominant gene controls the EP trait. Simple-sequence repeat (SSR) and bulked segregant analyses
of the F2 population revealed that the EP gene is located on chromosome 9, between two newly developed SSR markers, RM5833-11 and RM5686-23, at a genetic distance
of 1.5 and 0.9 cM, respectively. Markers closer to the EP gene were developed by amplified fragment length polymorphism (AFLP) analysis with 128 AFLP primer combinations. Three AFLP
markers were found to be linked to the EP gene, and the nearest marker, E-TA/M-CTC200, was mapped to the same location as SSR marker RM5686-23, 1.5 cM from the EP gene. A local map around the EP gene comprising nine SSR and one AFLP marker was constructed. These markers will be useful for marker-assisted selection
(MAS) for the EP trait in rice breeding programs. |
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Keywords: | AFLP EP gene Molecular markers SSR |
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