Molecular characterization of proteinaceous material in the Florida coastal Everglades |
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Authors: | Vera Jones Kathleen Parish Anu Thomson George A Wolff Nagamitsu Maie Rudolf Jaffé |
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Institution: | (1) Atkins Water, Chadwick House, Birchwood Science Park, Warrington, Cheshire, WA3 6AE, UK;(2) Southeast Environmental Research Center and Department of Chemistry, Florida International University, Miami, FL 33199, USA |
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Abstract: | Dissolved organic nitrogen (DON) is the least known component of the nitrogen cycle, in part as a result of the lack of adequate
analytical methods for its molecular characterization. In this study proteinaceous material in DON, collected at six geomorphologically
different sites in the Florida coastal Everglades, was characterized by amino acid analysis and protein gel electrophoresis.
The amino acid composition of the samples suggests that the canal DON was more degraded and subject to higher microbial inputs
than the mangrove marshwater and marine end-member stations. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
and two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) results supported this observation as distinctly different
protein profiles were obtained for the canal waters compared to samples collected at other stations. These preliminary results
highlight the potential of combining amino acid and intact protein analysis to fingerprint the sources of DON in different
aquatic environments, and show SDS-PAGE as a potentially useful method to characterize DON. |
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Keywords: | amino acids electrophoresis dissolved organic nitrogen microbial proteins |
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