全文获取类型
收费全文 | 3527篇 |
免费 | 526篇 |
国内免费 | 1262篇 |
出版年
2024年 | 9篇 |
2023年 | 197篇 |
2022年 | 209篇 |
2021年 | 253篇 |
2020年 | 249篇 |
2019年 | 262篇 |
2018年 | 256篇 |
2017年 | 234篇 |
2016年 | 228篇 |
2015年 | 197篇 |
2014年 | 235篇 |
2013年 | 387篇 |
2012年 | 198篇 |
2011年 | 221篇 |
2010年 | 191篇 |
2009年 | 218篇 |
2008年 | 196篇 |
2007年 | 220篇 |
2006年 | 207篇 |
2005年 | 147篇 |
2004年 | 128篇 |
2003年 | 109篇 |
2002年 | 88篇 |
2001年 | 90篇 |
2000年 | 81篇 |
1999年 | 63篇 |
1998年 | 42篇 |
1997年 | 51篇 |
1996年 | 48篇 |
1995年 | 48篇 |
1994年 | 41篇 |
1993年 | 37篇 |
1992年 | 25篇 |
1991年 | 29篇 |
1990年 | 22篇 |
1989年 | 18篇 |
1988年 | 15篇 |
1987年 | 7篇 |
1986年 | 8篇 |
1985年 | 10篇 |
1984年 | 10篇 |
1983年 | 4篇 |
1982年 | 11篇 |
1981年 | 4篇 |
1979年 | 5篇 |
1978年 | 1篇 |
1977年 | 2篇 |
1976年 | 1篇 |
1973年 | 1篇 |
1958年 | 1篇 |
排序方式: 共有5315条查询结果,搜索用时 187 毫秒
1.
Protected cultivation of ornamental flowers, as a commercial venture, becomes less profitable with excessive use of fertilizers. The present study examined the influence of microbial biofilm inoculants (Anabaena–Azotobacter, Anabaena–Trichoderma and Trichoderma–Azotobacter) on the availability of soil nutrients and structure of rhizosphere microbial communities in three varieties of chrysanthemum (var. White Star, Thai Chen Queen and Zembla). Varietal-specific responses in growth, enzyme activities, flower yield of plants and availability of soil nutrients were recorded. Dehydrogenase activity was highest in var. White Star treated with the Anabaena–Trichoderma biofilm inoculants. The Anabaena–Azotobacter inoculant enhanced the availability of nitrogen, phosphorus and micronutrients in the soil, besides 40–50% increase in soil organic carbon, as compared to carrier alone or no inoculation. PCR-DGGE profiling of the cyanobacterial communities and qPCR quantification of 16S rRNA abundance of bacteria, archaea and cyanobacteria in the rhizosphere soils, revealed the stronger influences of these inoculants, especially in var. Zembla. Principal Component Analysis (PCA) helped to illustrate that the enhanced microbe-mediated availability of soil macro-and micronutrients, except iron content (Fe), was the most influential factor facilitating improved plant growth and yield parameters. The Anabaena–Azotobacter, and Anabaena–Trichoderma biofilm inoculants, proved superior in all three chrysanthemum varieties. 相似文献
2.
3.
Larvae of the scarabaeid, Cyclocephala hirta, are major pests of turfgrass in California. A field test was conducted against third instars that included the following treatments: untreated control; chemical insecticide (bendiocarb); milky disease bacterium (Bacillus popilliae); and entomopathogenic nematodes (Steinernema feltiae and Heterorhabditis bacteriophora). There were no significant differences in population reduction among the treatments, but the larval population in all plots showed a dramatic decline. The C. hirta population had a natural occurrence of milky disease and blue disease caused by Rickettsiella popilliae. The prevalence of blue disease during the course of the study averaged < 10% but that of milky disease averaged about 20%. More significantly, the soil from all treatment plots when bioassayed for B. popilliae showed that 67–90% of the larvae became infected with this bacterium. None of the larvae became infected with the blue disease organism. We conclude that B. popilliae was occurring in epizootic proportions in our field tests and was a significant mortality factor in causing the decline of the C. hirta population. 相似文献
4.
A. J. Boulton 《Hydrobiologia》1991,211(2):123-136
Eucalypt leaf packs were placed at two sites in an intermittent stream during summer to examine the hypothesis that terrestrially-exposed leaf litter accumulates a richer microbial flora than submerged leaves — a phenomenon observed in Canadian temporary vernal pools. This did not occur; during the experiment, microbial biomass (as ATP) rose steadily on submerged leaves but remained low on terrestrially-exposed leaves. Densities of most functional feeding groups on the submerged leaves increased with time. Scrapers appeared to be more important than shredders in eucalypt leaf breakdown at both sites. 相似文献
5.
Soil architecture is the dominant control over microbially mediated decomposition processes in terrestrial ecosystems. Organic
matter is physically protected in soil so that large amounts of well-decomposable compounds can be found in the vicinity of
largely starving microbial populations. Among the mechanisms proposed to explain the phenomena of physical protection in soil
are adsorption of organics on inorganic clay surfaces and entrapment of materials in aggregates or in places inaccessible
to microbes. Indirect evidence for the existence of physical protection in soil is provided by the occurrence of a burst of
microbial activity and related increased decomposition rates following disruption of soil structures, either by natural processes
such as the remoistening of a dried soil or by human activities such as ploughing. In contrast, soil compaction has only little
effect on the transformation of 14C-glucose.
Another mechanism of control by soil structure and texture on decomposition in terrestrial ecosystems is through their impact
on microbial turnover processes. The microbial population is not only the main biological agent of decomposition in soil,
it is also an important, albeit small, pool through which most of the organic matter in soil passes.
Estimates on the relative importance of different mechanisms controlling decomposition in soil could be derived from results
of combined tracer and modelling studies. However, suitable methodology to quantify the relation between soil structure and
biological processes as a function of different types and conditions of soils is still lacking. 相似文献
6.
7.
8.
Lemuel B. Wingard Ching Hao Shaw James F. Castner 《Enzyme and microbial technology》1982,4(3):137-142
In the past 20 years, inorganic fuel cells have been transformed from novelty devices to practical energy transfer-energy storage units. However, the advantage of the high operating efficiency afforded by these fuel cells is partially offset by (a) the limited viability and high cost of the catalysts, (b) the highly corrosive electrolytes, and (c) the elevated operating temperatures. The possibility exists to reduce some of these problems through the development of bioelectrochemical fuel cells. Such biological/electrochemical systems incorporate either microorganisms or enzymes as an active component within the specified electrode compartments. Recent studies with microorganisms as part of the anode compartment have been aimed at defining the mechanism of the observed electrochemical reactions. Recent investigations on the use of cell-free enzyme preparations in the electrode compartments have dealt primarily with developing methodology and defining mechanisms for enhancing the rate of electron transfer from the enzyme-cofactor active site to the solid electrode surface. Applications of this developing technology have been envisioned for analytical chemistry, medical devices, energy transfer, electrochemical synthesis, and detoxification. In this review, the theory and problems of bioelectrochemical fuel cells are described and related to research, both recent and proposed, for the practical development of this area. 相似文献
9.
《Critical reviews in biotechnology》2013,33(4):374-381
Propionic acid (PA) is an important building block chemical and finds a variety of applications in organic synthesis, food, feeding stuffs, perfume, paint and pharmaceutical industries. Presently, PA is mainly produced by petrochemical route. With the continuous increase in oil prices, public concern about environmental pollution, and the consumers’ desire for bio-based natural and green ingredients in foods and pharmaceuticals, PA production from propionibacteria has attracted considerable attention, and substantial progresses have been made on microbial PA production. However, production of PA by propionibacteria is facing challenges such as severe inhibition of end-products during cell growth and the formation of by-products (acetic acid and succinic acid). The integration of reverse metabolic engineering and systematic metabolic engineering provides an opportunity to significantly improve the acid tolerance of propionibacteria and reduce the formation of by-products, and makes it feasible to strengthen the commercial competition of biotechnological PA production from propionibacteria to be comparable to the petrochemical route. 相似文献
10.
Kevin J. Beiler Suzanne W. Simard Valerie LeMay Daniel M. Durall 《Molecular ecology》2012,21(24):6163-6174
Understanding ectomycorrhizal fungal (EMF) community structure is limited by a lack of taxonomic resolution and autecological information. Rhizopogon vesiculosus and Rhizopogon vinicolor (Basidiomycota) are morphologically and genetically related species. They are dominant members of interior Douglas‐fir (Pseudotsuga menziesii var. glauca) EMF communities, but mechanisms leading to their coexistence are unknown. We investigated the microsite associations and foraging strategy of individual R. vesiculosus and R. vinicolor genets. Mycelia spatial patterns, pervasiveness and root colonization patterns of fungal genets were compared between Rhizopogon species and between xeric and mesic soil moisture regimes. Rhizopogon spp. mycelia were systematically excavated from the soil and identified using microsatellite DNA markers. Rhizopogon vesiculosus mycelia occurred at greater depth, were more spatially pervasive, and colonized more tree roots than R. vinicolor mycelia. Both species were frequently encountered in organic layers and between the interface of organic and mineral horizons. They were particularly abundant within microsites associated with soil moisture retention. The occurrence of R. vesiculosus shifted in the presence of R. vinicolor towards mineral soil horizons, where R. vinicolor was mostly absent. This suggests that competition and foraging strategy may contribute towards the vertical partitioning observed between these species. Rhizopogon vesiculosus and R. vinicolor mycelia systems occurred at greater mean depths and were more pervasive in mesic plots compared with xeric plots. The spatial continuity and number of trees colonized by genets of each species did not significantly differ between soil moisture regimes. 相似文献