玉米酯酶同工酶和过氧化物酶同工酶的分子量研究

本试验利用聚丙烯酰胺凝胶梯度电泳分步染色法直接对玉米苗期酯酶同工酶和过氧化物酶同工酶各酶带的分子量进行了比较测定。酯酶同工酶 E_1、E_2、E_3~F、E_3~S、a、b、c 各酶带的分子量分别为<20000,35200、33000、38500、29900、28500、34000道尔顿过氧化物酶同工酶 PX_4~F和 PX_4~S酶带的分子量分别为131000和149000道尔顿。根据酶带在均匀胶和梯度胶中的位置变化对各酶带的生化性质作了初步分析,发现 E_3~F和 E_3~S、PX_4~F 和 PX_4~S 在迁移率上的差异主要是分子量的差异。本文为同工酶的分子量测定提供了一个简便的方法。

STUDIES ON THE MOLECULAR WEIGHTS OF ESTERASE AND PEROXIDASE ISOZYMES OF MAIZE

The molecular weights of esterase and peroxidase isozymes of maize seedlings were directly determined by improved polyacrylamide gradient gel electrophoresis.The different isozyme bands developed in polyacrylamide slab gel electrophoresis(uniform gel)were identified in polyacrylamide gradient gel electrophoresis by means of isozyme variants.The molecular weights of esterase isozymes E_1,E_2,E_3~F,E_3~s,a,b,c,named according to isozyme patterns in uniform gel,are<20000,35200,33000,38500,29900,28500,34000 doltons respectively.The molecular weights of peroxidase isozymes PX_4~F and PX_4~s are 131000 and 149000 doltons respectively.Ac- cording to the band location in uniform gel and in gradient gel,some biochemical properties of the isozyme bands and relationships between the isozyme bands were analyzed.The possible er- rors in the determination of smaller molecular weight isozymes are discussed.