注入碳离子对甜菊的生物学效应

对甜菊（Ｓｔｅｖｉａ ｒｅｂａｕｄｉａｎｕｍ Ｂｅｒｔｏｎｉ）种子注入能量为７５ｋｅＶ、剂量为１０＾１４／ｃｍ＾２的碳离子，研究其种子萌发期产生的一系列生物学效应。注入碳离子的种子萌发率略高于对照组，但种苗的成活率比对照组低（Ｐ〈０．０２）。运用假设检验法分析，结果表明注入离子的种子出苗株高显著高于对照组（Ｐ〈０．０１）。萌发４ｄ的处理组，叶片细胞壁增厚，胞间连丝扩大，内有高电子工的物质沉积，细胞

Biological Effects of Stevia rebaudianum Induced by Carbon Ion Implantation

The biological effects during seed germination were investigated after the dry seeds of Stevia rebaudianum Bertoni were implanted with carbon ion beam of 75 keV and 10 14 ions/cm 2. The results showed that the germination rate of carbon ion implanted seeds was slightly higher than that of the control, but the survival rate of the treated seedlings, on the contrary, was lower than that of the control (P<0.02), while the height of the treated seedlings was significantly higher than that of the control (P<0.01). On the 4th day after germination, the leaf cell wall in the treated group was thick, some high electron_dense substance deposited in the enlarged plasmodesma; Cell membrane creased with high electron_dense granules deposited on it. The plasma membrane protruded towards cell wall, and the granules shifted via plasmodesma or deposited onto cell wall. These phenomena may be related to the conveyance of implanted ions across cell wall, or be related to the accumulation of callose. In addition, the implantation of carbon ions could increase the lamellae of the chloroplast and cause high development of the chloroplast which sometimes contained two plastid centers in an individual chloroplast. Also, the highly developed cristae, abundant mitochondria and typical crystalloid structure in microbody could be found. All these results indicated that the anabolic and catabolic activities in the seedlings implanted with carbon ions before germination were obviously more active than those in the controls.