| 基因组分析与小麦抗病育种 |
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| 引用本文： | 刘大钧.基因组分析与小麦抗病育种[J].Acta Botanica Sinica,2002,44(9):1096-1104. |
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| 作者姓名： | 刘大钧 |
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| 作者单位： | 南京农业大学细胞遗传研究所 南京210095 |
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| 基金项目： | 国家自然科学基金(A8610 4,A19412,39770 413)，国家“863”计划(Z17_0 4_01,BH_02_04_01)，国家科技部国际合作、攻关项目(C86350,B19112 )，农业部攻关、重点项目 (C863 21,C86327,B2 9111)l，教育部博士点基金(A2 8901,A2 9303)，江苏省自然科学基金，美国麦氏 |
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| 摘 要： | 系统总结了南京农业大学细胞遗传研究所近 2 0多年来利用基因组分析方法培育从簇毛麦 (Haynaldiavil losaSch .)、大赖草 (LeymusracemosusLam .)、鹅观草 (RoegneriakamojiC .Koch)和纤毛鹅观草 (R .ciliaris (Trin .)Nevs ki)导入白粉病和赤霉病抗性的小麦种质的研究进展。利用染色体C_分带、基因组原位杂交、分子标记 (特别是RFLP)等技术与非整倍体分析相结合对所创制的种质进行了系统分析与鉴定。还对所培育的小麦种质在育种实践和理论研究中的潜在价值及相关问题进行了讨论
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| 关 键 词： | 小麦 小麦近缘种 白粉病抗性 赤霉病抗性 染色体分带 原位杂交 分子标记 基因组分析 |
Genome Analysis in Wheat Breeding for Disease Resistance |
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| Authors: | LIU Da_Jun |
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| Abstract: | A brief review on the development of wheat germplasm with introduced powdery mildew and scab resistance from Haynaldia villosa Sch. and Leymus racemosus Lam., Roegneria ciliaris (Trin.) Nevski as well as R. kamoji C.Koch respectively was made. In the course of germplasm development, genome analysis by means of chromosome banding, genomic in situ hybridization (GISH) or fluorescence in situ hybridization (FISH), molecular markers, particularly restriction fragment length polymorphism (RFLP) coupled with aneuploid analysis was employed for the purpose of improving breeding efficiency. Potential use of such germplasm in wheat breeding practice, basic studies and some related problems were also discussed. |
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| Keywords: | Triticum aestivum wheat relatives powdery mildew resistance scab resistance chromosome banding in situ hybridization molecular marker genome analysis |
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