| 以谷胱甘肽为电子供体的细胞膜氧化还原系统 |
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| 引用本文： | 岳才军,陈珈.以谷胱甘肽为电子供体的细胞膜氧化还原系统[J].Acta Botanica Sinica,1997,39(5):433-438. |
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| 作者姓名： | 岳才军 陈珈 |
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| 作者单位： | 中国农业大学生物学院，中国农业大学生物学院 北京 100094，北京 100094 |
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| 基金项目： | 国家自然科学基金，高等院校博士学科点专项科研基金 |
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| 摘 要： | 内载谷胱甘肽(GSH)的大豆(Glycine max L.)下胚轴正向型质膜囊泡具有以GSH为电子供体的跨膜电子传递活性,能还原膜外电子受体FeCN和细胞色素(Cyt)C,其还原速率分别为(21.6±0.6)nmolFeCN·min~(-1)·mg~(-1)蛋白和(6.6±1.0)nmol Cyt C·min~(-1)·mg~(-1)蛋白。这种跨膜电子传递能引起膜上Cyt P-450吸收光谱标志带(Soret带)的变化,表明Cyt P-450参与了这一氧化还原过程。在跨质膜电子传递的同时伴随着H~ 运输和膜电位的改变。
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| 关 键 词： | 谷胱甘肽 细胞色素P-450 质膜氧化还原系统 电子传递 H~ 运输 膜电位 大豆下胚轴 质膜 |
REDOX SYSTEM OF PLASMA MEMBRANE WITH GLUTATHIONE AS AN ELECTRON DONOR |
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| Authors: | Yue Cai-jun and Chen Jia |
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| Abstract: | Glutathione(GSH)-loaded positive plasma membrane vesicles obtained by phase partitioning of microsomal fractions from soybean ( dycine max L.) hypocotyls were capable of transferring electrons from internal GSH to an externally added electron receptor, such as ferricyanide (FeCN) and cytochrome (Cyt) C. The rate of reduction for FeCN and Cyt C was (21.6 0.6) nmol FeCN min-1 mg-1 protein and (6.6 1.0) nmol Cyt C min-1 mg-1 protein,respectively. The transplasma membrane electron transport was able to change the distinct absorption spectra (Soret band) of Cyt P-450, which was widely present in the plant plasma membrane. This indicated that Cyt P-450 participated in the redox reaction. The transplasma membrane electron transport was associated with proton transport and the change of membrane potential. |
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| Keywords: | Glutathione Cytochrome P-450 Redox system in plasma membrane Electron transport Proton transport Membrane potential Soybean hypocotyl Plasma membrane |
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