普通菜豆种质资源芽期抗旱性鉴定

摘要：干旱是影响我国普通菜豆生产的主要因素之一，筛选芽期抗旱性种质资源，培育抗旱品种，有利于提高普通菜豆品种的出苗率和幼苗长势，对发展我国普通菜豆生产具有重要意义。本研究首先以4份普通菜豆种质为材料，检测了不同渗透势PEG6000溶液模拟旱胁迫下的发芽率和发芽势，确定了PEG6000溶液的最适渗透势为-0.7MPa（浓度为19.6%）；以-0.7MPa的PEG6000溶液对121份普通菜豆种质进行芽期模拟旱胁迫，测定发芽率、发芽势、下胚轴长、胚根长、干重和鲜重等10项指标；通过主成分分析筛选出相对发芽率、相对发芽势、相对鲜重、相对干重、相对胚根长，相对总芽长，相对胚根/下胚轴指数、相对发芽指数、相对活力指数等9项指标可以有效评价普通菜豆的芽期抗旱性；利用隶属函数分析法对121份种质的芽期抗旱性进行综合评价，筛选出跃进豆（F0000156）、白扁豆（F0000613）等芽期抗旱性种质，为普通菜豆抗旱生理与机制研究、抗旱育种奠定了基础。

Drought Resistance Identification of Common Bean Germplasm Resources at Bud Stage

Abstract: Drought is a major problem for common bean production and the drought tolerance in common bean is a priority research area in worldwide. The germination rate and germination vigor of common bean germplasm resources were evaluated with different PEG6000 density. The results showed that 19.6% (-0.7 MPa) is the optimum density for screening drought resources at bud stage of common bean. Thus, -0.7 MPa PEG6000 solution was used to analyze 121 accessions of common bean germplasm resources for drought resistance. Principal component analysis results showed that relevant germination rate (RGR), relevant germination vigor (RGV), relevant fresh weight (RFW), relevant dry weight (RDW), relevant root length (RRL), relevant shoot length (RSL), relevant root /hypocotyls (RR/H), relevant germination index (RGI) and relevant activity index (RAI) were considered as closely related traits for the drought resistance in common bean. Based on the membership function analysis, Yaojindou (F0000156) and Baibiandou (F0000613) exhibit high drought resistance.