盐地碱蓬GST基因的克隆、序列分析及其表达特征

从盐地碱蓬 (Suaedasalsa)幼苗的cDNA文库中克隆到一个 0 .9kb的全长cDNA ,同源性分析表明该全长cDNA与已报告的大豆 (Glycinemax)GST基因相应序列的同源性达 5 5 % ,可能编码由 2 35个氨基酸组成的谷胱甘肽转移酶 (glutathioneS transferase ,GST)。Southern杂交结果证明GST基因在碱蓬基因组中可能有至少两个以上的拷贝 ;Northern杂交结果表明 ,4 0 0mmol/L的NaCl处理 4 8h ,幼叶中GSTmRNA的表达量是对照的 2～ 3倍 ,说明碱蓬中GST基因受盐诱导

Cloning and Sequencing of GST Gene of Suaeda salsa and Its Expression Characteristics

A full length cDNA of about 0.9 kb was cloned from young seedlings of Suaeda salsa by constructing an cDNA library. According to the results of BLASTX homeologous analysis, the cDNA was shown to have 55% identification with the corresponding part of GST gene of Glycine max reported earlier. Sequencing analysis showed that the cDNA encodes glutathione S transferase enzyme consisted of 235 amino acids(Fig.1). Results of Southern blotting suggest that there may be more than two copies in genomic DNA of S. salsa (Fig.3) .Northern blotting shows that the relative expression levels under NaCl 400 mmol/L for 48 h are as 2-3 times as the control (Fig.4), which indicates GST gene in S.salsa is salt induced.