光敏核不育水稻农垦58S与其衍生不育系的叶绿体DNA的比较

扩增了光敏核不育系农垦58S及从它衍生出来的5个两用核不育系叶绿体DNA的ORF（open reading frame）100、ORF29-TrnC^GCA、rps16（ribosomal proteins16）基因内含子和TrnT^UGU-TrnL^UAA（tRNA^Thr（UGU）-tRNA^-Leu（UAA）等4个片段，并测定了其序列。研究结果表明，粳型光敏核不系农垦58S的叶绿体为粳型。农垦58S衍生的核不育系中，粳型核不育系7001S以及3个籼型核不育系1103S、培矮64S和广占63S的叶绿体DNA为粳型，与选育者提供的细胞质系谱一致。籼型核不育系W6154S的叶绿体DNA为籼型，与选育者提供的细胞质系谱不一致，推断选育者在选育过程中更换过细胞质（曾用不育系作过父本）。5个粳型叶绿体DNA的rps16基因内含子和TrnT^UGU-TrnL^UAA间区的序列相互之间有1-2个单核苷酸的变异。

Comparison of Chloroplast DNA between Photoperiod-sensitive Genic Male Sterile (PGMS) Rice Nongken58S and Its Derivative Sterile Lines

The ORF100, ORF29-TrnCGCA spacer, rps16 gene intron and TrnTUGU-TrnLUAA spacer of chloroplast DNA (cpDNA) of 6 photoperiod-sensitive genic male sterile (PGMS) rice, Nongken58S and its 5 derivatives, were amplified and sequenced. According to the result of ORF100 and ORF29-TrnCGCA spacer analysis, the cpDNA of japonica PGMS line Nongken58S was Japonica, and among those PGMS lines derived from Nongken58S, cpDNA of japonica 7001S and 3 indica lines 1103S, Peiai64S and Guangzhan63S were that of japonica, which was in accordance with the cytoplasm pedigree provided by their breeders. But the cpDNA of indica PGMS line W6154S was that of indica, which disaccorded with the cytoplasm pedigree, so we conjec- tured that the breeders had used the PGMS line as the male parent. Basing on the result of sequence analysis, we found single nucleotide polymorphism in rps16 gene intron and TrnTUGU-TrnLUAA spacer of these 5 japonica PGMS lines.