| RNA干扰在原生动物中的应用 |
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| 引用本文： | 曾红,沈洁,顾福康.RNA干扰在原生动物中的应用[J].细胞生物学杂志,2006,28(2):179-182. |
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| 作者姓名： | 曾红 沈洁 顾福康 |
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| 作者单位： | 华东师范大学生命科学学院，福建师范大学生命科学学院 福州350007，华东师范大学生命科学学院，华东师范大学生命科学学院，上海200062，上海200062，上海200062 |
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| 基金项目： | 国家自然科学基金资助项目(No.30470201)~~ |
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| 摘 要： | RNA干扰(RNAi),即由双链RNA介导的转录后基因沉默的现象,已成为分析原生动物基因功能的有效手段。现对应用RNAi研究原生动物微管蛋白的功能和基体组装、纤毛虫大核基因组重排、纤毛虫刺丝泡的发生和作用、端粒酶及细胞周期蛋白的功能等方面进行综述。
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| 关 键 词： | 原生动物 RNA干扰 双链RNA 基因功能 |
| 收稿时间： | 2005-07-11 |
| 修稿时间： | 2005-12-13 |
The Application of RNA Interference to Protozoa |
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| Hong Zeng,Jie Shen,Fu-Kang Gu.The Application of RNA Interference to Protozoa[J].Chinese Journal of Cell Biology,2006,28(2):179-182. |
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| Authors: | Hong Zeng Jie Shen Fu-Kang Gu |
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| Institution: | 1 School of Life Sciences, East China Normal University, Shanghai 200062, China; 2College of Life Sciences, Fujian Normal University, Fuzhou 350007, China |
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| Abstract: | RNA interference (RNAi), triggered by double stranded RNA (dsRNA), was the post-transcrip-tional gene silencing (PTGS) based on sequence-specific degradation of mRNA. It had been proved to be an effi-cient method for analyzing gene functions in protozoa. In this paper, we reviewed the RNAi methods and their mainresearch progress in cell and molecular biology of protozoa which focused on the assembly of base body andfunctions of tubulins, cyclins and telomerase, gene rearrangement and exocytosis of the trichocyst of the ciliatedprotozoa. |
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| Keywords: | RNA interference double stranded RNA protozoa gene function |
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