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1.
With many advantages, many ecological parameters of protozoan communities have been successfully used as a useful bioindicator for bioassessment of water quality in Chinese marine waters. However, as regard the response of the annual cyclicity of protozoan communities to seasonal environmental stress, a further investigation was needed. In this study, the cyclicity of annual variations in community patterns of biofilm-dwelling protozoa was studied based on an annual dataset. Samples were monthly collected, using glass slide method, at four stations, within a pollution gradient, in coastal waters of the Yellow Sea, northern China during a 1-year period. The cyclicity patterns of the microbiota represented a significant spatial variation among four stations. The low value of cyclicity coefficients occurred in heavily polluted area, while the high values were in less stressed areas. Correlation analysis showed that the cyclicity measure was significantly related to environmental variables ammonia, transparency and dissolved oxygen. Thus, it is suggested that the annual cyclicity of protozoan communities may be used as a potential bioindicator of bioassessment in marine ecosystems. 相似文献
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F. Hubert M.-H. Rodier A. Minoza V. Portet-Sulla E. Cateau K. Brunet 《Letters in applied microbiology》2021,72(1):82-89
Candida auris is an emerging species responsible for life-threatening infections. Its ability to be resistant to most systemic antifungal classes and its capacity to persist in a hospital environment have led to health concerns. Currently, data about environmental reservoirs are limited but remain essential in control of C. auris spread. The aim of our study was to explore the interactions between C. auris and two free-living amoeba (FLA) species, Vermamoeba vermiformis and Acanthamoeba castellanii, potentially found in the same water environment. Candida auris was incubated with FLA trophozoites or their culture supernatants. The number of FLA and yeasts was determined at different times and transmission electron microscopy (TEM) was performed. Supernatants of FLAs promoted yeast survival and proliferation. Internalization of viable C. auris within both FLA species was also evidenced by TEM. A water environmental reservoir of C. auris can therefore be considered through FLAs and contamination of the hospital water networks would consequently be possible. 相似文献
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Yalitza Lopez Corcino Shekina Gonzalez Ferrer Luz Eliana Mantilla Sophia Trikeriotis Jin‐Sang Yu Steven Kim Samuel Hansen Jose‐Andres C. Portillo Carlos S. Subauste 《Cellular microbiology》2019,21(10)
Toxoplasma gondii causes retinitis and encephalitis. Avoiding targeting by autophagosomes is key for its survival because T. gondii cannot withstand lysosomal degradation. During invasion of host cells, T. gondii triggers epidermal growth factor receptor (EGFR) signalling enabling the parasite to avoid initial autophagic targeting. However, autophagy is a constitutive process indicating that the parasite may also use a strategy operative beyond invasion to maintain blockade of autophagic targeting. Finding that such a strategy exists would be important because it could lead to inhibition of host cell signalling as a novel approach to kill the parasite in previously infected cells and treat toxoplasmosis. We report that T. gondii induced prolonged EGFR autophosphorylation. This effect was mediated by PKCα/PKCβ ? Src because T. gondii caused prolonged activation of these molecules and their knockdown or incubation with inhibitors of PKCα/PKCβ or Src after host cell invasion impaired sustained EGFR autophosphorylation. Addition of EGFR tyrosine kinase inhibitor (TKI) to previously infected cells led to parasite entrapment by LC3 and LAMP‐1 and pathogen killing dependent on the autophagy proteins ULK1 and Beclin 1 as well as lysosomal enzymes. Administration of gefitinib (EGFR TKI) to mice with ocular and cerebral toxoplasmosis resulted in disease control that was dependent on Beclin 1. Thus, T. gondii promotes its survival through sustained EGFR signalling driven by PKCα/β ? Src, and inhibition of EGFR controls pre‐established toxoplasmosis. 相似文献
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Anna Stincone Alessandro Prigione Thorsten Cramer Mirjam M. C. Wamelink Kate Campbell Eric Cheung Viridiana Olin‐Sandoval Nana‐Maria Grüning Antje Krüger Mohammad Tauqeer Alam Markus A. Keller Michael Breitenbach Kevin M. Brindle Joshua D. Rabinowitz Markus Ralser 《Biological reviews of the Cambridge Philosophical Society》2015,90(3):927-963
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三峡水库运行期间原生动物群落的时空异质性 总被引:1,自引:0,他引:1
于2010年10月—2011年6月三峡水库正常运行周期内对库区干流原生动物进行调查,研究其空间分布及水库周期排蓄期间的变化。共检测到原生动物99种,蓄水后纤毛虫有增多的趋势。水库运行的不同阶段优势种不同,大致演变为:砂壳纤毛虫(蓄水期)—非砂壳类纤毛虫(高位运行期间)—有壳肉足虫(低位运行期间)。不同水域优势种也存在差异,从上游到下游特征指示种变化为:有壳肉足虫(变动回水区)—纤毛虫(湖泊区)。结果表明,三峡水库原生动物的分布具有明显的时空异质性(P0.05),影响原生动物时空分布的主要因素有透明度、温度、电导率和叶绿素a。原生动物平均密度为952.19个/L,平均生物量为8.14μg/L。蓄水期上游现存量高于下游,低位运行期间则低于下游。原生动物Marglef和Shannonn-Weiver多样性指数平均值分别为3.78和2.18,1月份最低,6月份最高。蓄水175 m后上游变动回水区原生动物具有较高的丰度。水库冬蓄夏排的运行模式模糊了河流本身的季节变化,使原生动物的种类和现存量更多的受水库水动力学的影响,使水体理化因子和水文特征呈现明显的时空差异,最终形成原生动物种群的时空异质性分布。 相似文献
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Jacqueline W.A. Scheepmaker Jan van de Kassteele 《Biocontrol Science and Technology》2011,21(10):1225-1242
Our aim was to investigate the non-target effects on soil micro-organisms in agricultural environments caused by chemical control agents (CCAs) and microbial biocontrol agents (mBCAs), including the recovery from these effects. This was a desk study in which quantifiable endpoints, such as numbers of colony forming units (CFU), were derived from a series of studies and the combined data then analysed with a meta-regression analysis. Three analyses of the same dataset were performed. The first analysis, which was performed at the level of the CCAs and mBCAs in general, revealed that the effects of CCAs differed significantly from those of mBCAs. The second analysis, which included the type of non-target group (bacteria, fungi, actinomycetes, and protozoa) as additional input, revealed that mBCAs have greater effects than CCAs on fungi at study initiation, that CCAs had greater effects than mBCAs on bacteria and protozoa and that when effects were measured, recovery occurred within 100 days post-treatment initiation. The final analysis, which included the type of CCA (fungicide, insecticide, herbicide) or mBCA (antagonist) as additional input, revealed that (1) antagonists had a greater effect on fungi than insecticides and fungicides, (2) insecticides and to a lesser extent fungicides had a larger effect on bacteria than fungicides and antagonists, and (3) recovery of the CFU occurred within 100 days for all types of pesticides, mBCAs as well as CCAs, and for all non-target groups. The findings are discussed in view of the regulatory context of admittance of mBCAs to the market. 相似文献