| 差异表达基因的高通量筛选方法 |
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| 引用本文： | 李斐雪,王雁玲.差异表达基因的高通量筛选方法[J].细胞生物学杂志,2004,26(4):339-343. |
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| 作者姓名： | 李斐雪 王雁玲 |
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| 作者单位： | 中国科学院动物研究所计划生育生殖生物学国家重点实验室，中国科学院动物研究所计划生育生殖生物学国家重点实验室 北京 100080，北京 100080 |
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| 摘 要： |
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| 关 键 词： | 差异表达基因 细胞群 cDNA阵列 抑制消减杂交技术 基因芯片技术 |
| 修稿时间： | 2003年9月30日 |
High Throughput Screening Methods Used to Identify Differentially Regulated Genes |
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| Fei-Xue Li,Yan-Ling Wang.High Throughput Screening Methods Used to Identify Differentially Regulated Genes[J].Chinese Journal of Cell Biology,2004,26(4):339-343. |
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| Authors: | Fei-Xue Li Yan-Ling Wang |
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| Abstract: | cDNA array, gene chip, iAFLP (introduced AFLP), SAGE (serial analysis of gene expression), cDNA-AFLP, DDRT-PCR, RC4D (RFLP-coupled differential display) and SSH (suppression subtractive hybridization) are techniques being used extensively to identify differentially regulated genes in organisms. In this review, these display systems are evaluated and compared. The general principles on which the different techniques are based and which determine their potential and their limitations are described. Some examples are given about the utilization of these methods. |
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| Keywords: | differentially expressed gene high throughput Screening SAGE DDRT-PCR SSH |
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