栀子道地性的分子生态学

运用扩增片段长度多态性(AFLP)方法，研究了来自江西5个不同产地栀子的亲缘关系，并运用HPLC方法测定了栀子苷的含量，用ICP法测定了植物药材中的矿质元素含量.结果表明，各地方品系间在DNA 水平上存在明显的多样性变异;运用NTSYS-PC 软件对53个个体聚类分析，地理位置相近的种群聚为一类.各样本间栀子苷含量高低与聚类分支间无明显相关性，说明道地性的产生是基因型和环境饰变共同作用的结果.经微量元素测定,锌与栀子苷含量间呈负相关关系.

Molecular ecology of Gardenia jasminoides authenticity

By using amplified fragment length polymorphism (AFLP) markers, this paper studied the genetic relationships among five wild or cultivated Gardenia jasminoides Ellis populations in Jiangxi Province. Chemical fingerprint was also built with HPLC method. The results showed that there was a great genetic difference among these samples. The UPGMA obtained with NTSYS-PC 2.10e software suggested that there were seven branches of population, and the population from near geographical location clustered firstly. The geniposide content of these branches was not correlated with UPGMA. It could be concluded that the authenticity was resulted from the co-action of genotype and environmental change. The microelements content in G. jasminoides fruit measured by inductively coupled plasma showed that there was a negative correlation between Zn and geniposide contents.