| 2,4,6－三氯苯酚诱导鲫鱼肝脏自由基的产生及其氧化应激 |
| |
| 引用本文： | 纪靓靓,李法云,罗义,马溪平,陈忠林.2,4,6－三氯苯酚诱导鲫鱼肝脏自由基的产生及其氧化应激[J].应用生态学报,2007,18(1):129-132. |
| |
| 作者姓名： | 纪靓靓 李法云 罗义 马溪平 陈忠林 |
| |
| 作者单位： | 1. 辽宁大学资源与环境学院,沈阳,110036
2. 南开大学环境科学与工程学院,天津,300071 |
| |
| 基金项目： | 国家重点基础研究发展计划(973计划);国家自然科学基金;辽宁省环境保护开放实验室基金 |
| |
| 摘 要： | 采用电子顺磁共振的方法，研究了鲫鱼腹腔注射2,4,6-三氯苯酚(2,4,6-TCP)不同时间(4、8、12、24、72 h)后其肝脏自由基强度的变化、氧化应激反应及其损伤机理.结果表明：2,4,6-TCP极显著促进了鲫鱼肝脏自由基的产生；鲫鱼肝脏内超氧化物歧化酶 (SOD) 与谷胱甘肽硫转移酶 (GST) 的活性受到显著诱导 ，过氧化氢酶 (CAT) 活性受到抑制，还原型谷胱甘肽 (GSH) 含量与对照组无明显差异，氧化型谷胱甘肽 (GSSG) 含量显著升高 ，丙二醛(MDA) 含量极显著增加.
|
| 关 键 词： | 2 4 6-三氯苯酚 自由基 鲫鱼 氧化应激 电子顺磁共振 |
| 文章编号： | 1001-9332(2007)01-0129-04 |
| 收稿时间： | 2006-3-16 |
| 修稿时间： | 2006-03-16 |
Free radicals in Carassius auratus liver:Their generation and oxidative stress induced by 2,4,6-trichlorophenol |
| |
| JI Liang-liang,LI Fa-yun,LUO Yi,MA Xi-ping,CHEN Zhong-lin.Free radicals in Carassius auratus liver:Their generation and oxidative stress induced by 2,4,6-trichlorophenol[J].Chinese Journal of Applied Ecology,2007,18(1):129-132. |
| |
| Authors: | JI Liang-liang LI Fa-yun LUO Yi MA Xi-ping CHEN Zhong-lin |
| |
| Institution: | 1School of Resources and Environment, Liaoning University, Shenyang 110036, China; 2College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China |
| |
| Abstract: | With electron paramagnetic resonance(EPR)technique,this paper studied the free radicals generation and oxidative stress in Carassius auratus liver after 4,8,12,24 and 72 hours of 2,4,6-trichlorophenol(2,4,6-TCP)intraperitoneal injection.The results showed that 2,4,6-TCP significantly promoted the generation of free radicals in C.auratus liver.The superoxide dismutase(SOD)and glutathione-S-transferase(GST)activities were induced significantly,while catalase(CAT)activity was inhibited,and glutathione(GSH)content had little difference with the control.A significant increase was observed in the contents of oxidized glutathione(GSSG)and malondialdehyde(MDA). |
| |
| Keywords: | 2 4 6-trichlorophenol free radical Carassius auratus oxidative stress electron paramagnetic resonance |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《应用生态学报》浏览原始摘要信息 |
| 点击此处可从《应用生态学报》下载免费的PDF全文 |
|
