A radioimmunoassay of plasma estradiol-17beta with the use of polyethylene glycol to separate free and antibody-bound hormone |
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Authors: | H S Schiller M A Brammall |
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Institution: | From the Departments of Laboratory Medicine and Obstetrics and Gynecology University of Washington, Seattle, Washington 98195 USA |
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Abstract: | A radioimmunoassay for plasma estradiol-17β was developed using polyethylene glycol to separate free from antibody-bound hormone. Specificity for estradiol-17β was achieved by a modified celite microcolunm procedure in which estradiol was.separated from interfering estrogens, including estrone. Using trace 3H-estradiol to monitor procedural losses, the method was shown to be sensitive and accurate. Intra- and inter-assay coefficient of variation of the method was 8.7 and 10.6%, respectively. Polyethylene glycol used for antibody precipitation appears to be a generally applicable method for steroid hormone radioimmunoassays. The simplicity, precision and rapid analysis, coupled with its lack of time dependence and ease in automation, makes this a convenient and practical method. |
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Keywords: | B A B 0 1 M barbital acetate buffer (pH 7 0) R I A radioimmunoassay estradiol-17β 1 3 5 (10)-estratrien-3 17β-diol estrone 3-hydroxy 1 3 5 (10)-estratrien-17-one peg polyethylene glycol |
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