西门塔尔牛产奶性状的微卫星标记分析

通过对西门塔尔牛育种核心群6个父系组成的150头母牛产奶性状的测定，结合微卫星标记技术，于4条染色体上选择12个微卫星位点进行产奶性状的微卫星标记分析，研究各位点对乳脂、乳蛋白、乳糖、干物质和奶中体细胞数的遗传效应。结果表明： 12个位点都具有高度多态性，杂合度（H）在0.64～0.86之间，多态信息含量（PIC）也达到0.60以上，最高者ILST093的多态信息含量为0.85；位点ILST093对奶中体细胞数有显著性影响(P<0.05)，位点BMS711对乳脂率有显著性影响(P<0.05)，位点BM1905与奶中乳糖含量呈显著相关(P<0.05)，位点BM6438与五个产奶性状均无相关性。

Analysis of microsatellite markers with the milk product traits in Simmental

12 microsatellite markers from 4 chromosomes were chosen to analyze the effect on 5 milk production traits (including fat, lactoprotein, lactose, dry matter content and somatic cell number) of 150 cows from a 6-male-line Simmental breeding core group. Results showed that the 12 microsatellite markers were highly polymorphic and their PIC (polymorphism information contents) were over 0.60, and the marker with the highest PIC (0.85) was ILST093. The heterozygosities were among 0.64 and 0.86. Effects of 12 microsatellite loci on milk production traits were analyzed. Results showed that ILST093 had a significant effect on somatic cell number (P<0.05). Effects of BMS711 on fat ratio and of BMS1905 on lactose both reached a significant level (P<0.05), BM6438 on milk production traits had no significant effect.