| 香猪肌肉组织cDNA文库的构建及其EST测序成功率的分析 |
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| 引用本文： | 王秀利,李宁,赵志辉,冯继东,赵兴波,李长绿,吴常信.香猪肌肉组织cDNA文库的构建及其EST测序成功率的分析[J].遗传,2002,24(3):263-266. |
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| 作者姓名： | 王秀利 李宁 赵志辉 冯继东 赵兴波 李长绿 吴常信 |
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| 作者单位： | 1．中国农业大学动物科技学院，北京100094；
2．中国农业大学农业生物技术国家重点实验室，北京100094；
3．黑龙江八一农垦大学动物科技学院， 密山 158308
1．College of Animal Science and Technology,China Agricultural University,Beijing 100094,China;
2．National Laboratory for Agrobiotechnology of China Agricultural University,Beijing 100094,China;
3．College of Animal Science and Technology,Heilongjiang August First Land Reclamation University,Mishan 158308,China |
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| 基金项目： | 国家重大基础研究发展规划项目 973项目 (G2 0 0 0 0 16 1)，国家自然科学基金重点项目 ( 39730 36 0 ) |
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| 摘 要： | 以香猪背最长肌为实验材料，以Lambda ZAP II为载体，构建了肌肉组织的cDNA文库。结果表明，cDNA文库的滴度在3.4×107 pfu/ml以上，重组率在94%以上，插入片段大小平均在1.5kb以上。同时指出，如果从3′端测序，多于30 个碱基T的插入片段是造成ESTs测序成功率低的主要因素。
Abstract:Using Longissimus Dorsi muscle as material and Lambda ZAP II as Vector,Xiang Pig Longissimus Dorsi muscle cDNA library has been constructed in our study.The results showed that the tritation of the library was 3.4×107 pfu/ml，the recombinant percentage was 94%，and the fragment length of inserted average cDNA were 1.5kb.The study pointed out that the more than 30 T insertion is the major factor for low percetage if sequencing the 3′-end.
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| 关 键 词： | 香猪 背最长肌 xiang pig ESTs Key words cDNA文库 |
| 文章编号： | 0253-9772(2002)03-0263-04 |
| 修稿时间： | 2002年2月5日 |
Analysis of Succeed Percentage on Sequencing ESTs and Construction of Porcine Muscle cDNA library |
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| WANG Xiu li ,LI Ning ,ZHAO Zhi hui ,FENG Ji dong ZHAO Xing bo ,LI Chang lv ,WU Chang xin.Analysis of Succeed Percentage on Sequencing ESTs and Construction of Porcine Muscle cDNA library[J].Hereditas,2002,24(3):263-266. |
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| Authors: | WANG Xiu li LI Ning ZHAO Zhi hui FENG Ji dong ZHAO Xing bo LI Chang lv WU Chang xin |
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| Institution: | College of Animal Science and Technology, China Agricultural University, Beijing 100094, China. |
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| Abstract: | Using Longissimus Dorsi muscle as material and Lambda ZAP II as Vector, Xiang Pig Longissimus Dorsi muscle cDNA library has been constructed in our study. The results showed that the titration of the library was 3.4 x 10(7) pfu/ml, the recombinant percentage was 94%, and the fragment length of inserted average cDNA were 1.5 kb.The study pointed out that the more than 30 T insertion is the major factor for low percentage if sequencing the 3'-end. |
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| Keywords: | xiang pig longissimus dorsi muscle cDNA library ESTs |
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