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| 中间球海胆、光棘球海胆及杂交F1代(中间球海胆♀×光棘球海胆♂)群体遗传多样性AFLP分析 | |
| 引用本文： | 周遵春,包振民,董颖,刘相全,宋伦,赫崇波,王丽梅.中间球海胆、光棘球海胆及杂交F1代(中间球海胆♀×光棘球海胆♂)群体遗传多样性AFLP分析[J].遗传,2007,29(4):443. |
| 作者姓名： | 周遵春 包振民 董颖 刘相全 宋伦 赫崇波 王丽梅 |
| 作者单位： | 1. 辽宁省海洋水产科学研究院,辽宁省海洋水产分子生物学重点实验室,辽宁省应用海洋生物技术开放实验室,大连,116023 2. 中国海洋大学,海洋生命学院,青岛,266003 3. 山东省海洋水产研究所,烟台,264006 |
| 基金项目： | 国家高技术研究发展计划项目(国家863计划)(编号:2006AA10A411)，辽宁省自然科学基金项目(编号:20042127)，大连市优秀青年科技人才基金项目(编号:S2006J23JH029)资助~~ |
| 摘 要： | 应用AFLP技术对中间球海胆、光棘球海胆及杂交F1代(中间球海胆♀×光棘球海胆♂)群体的遗传多样性进行了分析。结果表明, 4对引物共扩增得到272个位点, 其中269个多态位点, 总的多态位点比例为98.89%。3个群体的香农多样性指数分别为: 0.2331 ± 0.1273、0.2005 ± 0.1385和0.2625 ± 0.1067。群体内遗传相似度分别为: 0.6876 ± 0.0523、0.6501 ± 0.0548和0.6552 ± 0.0553。分子方差分析(AMOVA)结果表明, 变异来源有25.39%来自群体间, 有74.61%来自群体内, 群体内的遗传多样性比较丰富。尽管杂交海胆在表型上可以明显分成两种类型, 但是通过AFLP统计的遗传距离进行的个体聚类却随机聚在一起, 不能分成两个群体。 |
| 关 键 词： | 光棘球海胆 中间球海胆 杂交 AFLP |
| 收稿时间： | 2006-08-01 |
| 修稿时间： | 2006-11-06 |
AFLP analysis in populations of Strongylocentrotus intermedius,S. nudus and hybrids (S. intermedius ×S. nudus) |
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| ZHOU Zun-Chun,BAO Zhen-Min,DONG Ying,LIU Xiang-Quan,SONG Lun,HE Chong-Bo,WANG Li-Mei.AFLP analysis in populations of Strongylocentrotus intermedius,S. nudus and hybrids (S. intermedius ×S. nudus)[J].Hereditas,2007,29(4):443. | |
| Authors: | ZHOU Zun-Chun BAO Zhen-Min DONG Ying LIU Xiang-Quan SONG Lun HE Chong-Bo WANG Li-Mei |
| Institution: | 1. Liaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Open Lab of Applied Marine Biotechnology, Dalian 116023, China; 2. College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; 3. Shandong Fishery Institute, Yantai 264006, China |
| Abstract: | AFLP analysis of genetic diversity in the three populations of sea urchin Strongylocentrotus intermedius (IN), S. nudus (NU) and F1 progeny (IN×NU) was carried out in this paper. In total, 272 loci were amplified with 4 primer pairs, of which 269 were polymorphic and the percentage of polymorphic loci was 98.89%. The Shannon diversity index for S. intermedius, S. nudus, and their hybrid populations was 0.2331±0.1273, 0.2005±0.1385, and 0.2625±0.1067, respectively. The genetic similarity within populations was 0.6876±0.0523, 0.6501±0.0548, and 0.6552±0.0553, respectively. AMOVA analysis indicated that 25.39% of variance was among populations and 74.61% of variance was within populations. This suggested the rich genetic diversity level within populations. Although the hybrids can be classified into two types by apparent characters, they were clustered each other by UPGMA method according to their genetic distances. |
| Keywords: | Strongylocentrotus nudus S intermedius hybrids AFLP |
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