| 特异性PCR反应鉴别携带W染色体的嵌合体公鸡子代性别的研究 |
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| 引用本文： | 燕海峰,肖兵南,吴晓林.特异性PCR反应鉴别携带W染色体的嵌合体公鸡子代性别的研究[J].遗传,2001,23(4):306-308. |
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| 作者姓名： | 燕海峰 肖兵南 吴晓林 |
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| 作者单位： | 1。湖南省畜牧兽医研究所，长沙 410131
2.Research Institute of Biopharmacy and Veterinary Drugs,25449 Jilove,Prague,Czech Republic
1.Hunan Institute of Animal and Veterinary Science,Changsha 410131,China;
2.Research Institute of Biopharmacy and Veterinary Drugs,25449 Jilove,Prague,Czech Republic |
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| 基金项目： | 本研究为中捷政府间科技合作项目(中方编号:98-41-22;捷方编号:GACR No.523-98-1607)的部分研究内容 |
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| 摘 要： | 利用聚合酶链式反应(PCR)特异扩增家鸡W染色体高度重复序列EcoRI家族的447bp片段,作为鉴定家鸡W染色体存在的依据.4个表现型为雄性的嵌合体公鸡的精液中检测到了W染色体,通过人工授精获得嵌合体子一代.检测了其中188个雏鸡的血液,51.6%(97/188)含有W染色体,x2检验结果显示,雌雄后代符合1:1的比例,这表明在这188个成活的嵌合体子代中,基本上没有W染色体精子所产生的雏鸡,携带W染色体的精子没有将其W染色体传递到其子代雏鸡中.本研究利用PGR性别鉴定技术,从分子水平上对精液中携带W染色体的嵌合体公鸡(ZW→ZZ型)子代的性别分化作了初步的探讨。
Abstract:The W chromosome was detected in four cocks' sperms by a method based on the polymerase chain reaction which amplifies the EcoRI repeat unit of the chicken W chromosome.188 offsprings of these chimeric cocks were obtained by artificial insemination and 51.6% (97/188) was detected W chromosome in blood.The result of χ2 test suggests that the sex ratio is 1:1.It seems as if no evidence was found for skewed sex ratios of these offsprings.Therefore it seems as if the sperms with W chromosome could not contribute to these 188 offspring chicks.
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| 关 键 词： | 嵌合体鸡 W染色体 PCR 性别鉴定 Key words PCR |
| 文章编号： | 0253-9772(2001)04-0306-03 |
| 修稿时间： | 2000年7月19日 |
Sex Determination of Offspring of the Chimeric Cocks Carrying a W Chromosome Based on Specific PCR |
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| YAN Hai feng ,P.Trefil ,XIAO Bing nan ,WU Xiao lin.Sex Determination of Offspring of the Chimeric Cocks Carrying a W Chromosome Based on Specific PCR[J].Hereditas,2001,23(4):306-308. |
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| Authors: | YAN Hai feng PTrefil XIAO Bing nan WU Xiao lin |
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| Institution: | YAN Hai feng 1,P.Trefil 2,XIAO Bing nan 1,WU Xiao lin 1 |
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| Abstract: | |
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| Keywords: | PCR chimeric chickens W chromosome sex discrimination |
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