利用RAPD标记构建美洲黑杨×欧美分子标记连锁图谱

本文利用RAPD标记和美洲黑杨(Populus deltoides)×欧美杨(P.euramericana)的F1群体，构建了美洲黑杨×欧美杨的分子标记连锁图谱。实验过程中对1040个寡核苷酸随机引物进行了重复筛选，共选出127个引物用于作图群体(包括双亲共92个无性系)的随机扩增，这127个引物产生229个多态基因座，其中符合“拟测交”1∶1分离的有214个。利用多点连锁分析，形成19个连锁群及6个三连体和14个连锁对。由19个连锁群构成的图谱含标记129个，总图距为1914.2cM，覆盖杨树基因组约73.62％。标记间的平均间距为14.84cM。本研究获得了中等密度的美洲黑杨×欧美杨的一个连锁框架。 Abstract:A molecular linkage map was constructed for the parents of a P.deltoides × P.euramericana F1 family based on random amplified polymorphic DNA(RAPD)markers.A set of 1040 random oligonucleotide primers were screened and 127 primers were selected to generate RAPD markers within a sample of 90 F1 progenies.A total of 229 segregating loci were identified.Among the 229 loci,15 loci were found distorted from the normal 1∶1 ratio.Using multiple analysis,the 214 markers formed 19 main Linkage groups (including 129 markers)and b triples and 14 pairs.The resulting Linkage map of Populus deltoides × P.euramericana (including 129 markers)spanned 1914.2cM(73.62％ coverage of genome length)with an average distance of 14.84cM between markers.