西双版纳小耳猪DNA指纹分析

采用ECL核酸直接标记和检测试剂盒,以HRP标记JL-02多位点探针,对云南版纳小耳猪的HinfI或HaeⅢ酶切图谱进行Southern杂交,以化学发光法进行检测,获得了清晰可辨的、具有高度多态性的DNA指纹图谱.大于2kb的谱带数在13～17条之间,JB亚系内805、807家系和JS亚系内111、121、133、151家系不同个体间的相似系数分别为0.92±0.04、0.85±0.13、0.86±0.02、0.80±0.05、0.84±0.09、0.88士0.08,显著高于家系间的相似系数.JB亚系内的805家系和JS亚系内的151家系不同个体间的相似系数最大,分别为0.92和0.88,说明其近交程度较高,个体间差异较小,均一性较强.另外,JB亚系内的805与807两个家系不同个体猪在11.5kb处,均有一条区别于其他家系的特有图带,其是否是导致JB亚系体型较大的特异性基因座,有待进一步研究。 Abstract:Clearly and highly polymorphic DNA fingerprints from Banna miniature pig were obtained by Southern hybridization with a nonisotopieally HRP(Horseradish peroxidase)labelled multiloci minisatellite probe JL-02,the fragment number of which were ranged from 13 to 17.The similarity coefficient of various individuals from family “805”,“807” in substrain JB and family “111”,“121”,“133”,“151” in substrain JS was 0.92 + 0.04,0.85 + 0.13,0.86 +- 0.02,0.80 +0.05,0.84 + 0.09,0.88 + 0.08,which is higher than that bet ween families(0.46~0.65).Furtherly,similarity coefficients of family “805” in substrain JB and family “151” in substrain JS were 0.92 and 0.88 respectively.This showed that the two families have the higher inbreeding degree,smaller genetic difference,among individuals and the better homogeneity.In addition,all individuals from family 805,807 in substrain JB have a unique fragment different from other individuals from other families,which is abount 11.5kb long.This suggested that this may be the gene resulted in large bodyweight of substrain JB,further research is necessary to be conducted.

DNA Fingerprinting for Banna Miniature Pig