| 鸡种系嵌和体的研制及其AFLP检测 |
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| 引用本文： | 胡小芬,谢蓓,于瑞嵩,黄启忠,张德福,黄路生,李震.鸡种系嵌和体的研制及其AFLP检测[J].遗传,2005,27(3):367-371. |
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| 作者姓名： | 胡小芬 谢蓓 于瑞嵩 黄启忠 张德福 黄路生 李震 |
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| 作者单位： | 1.上海市农业科学院畜牧兽医研究所，上海，201106;2.上海市农业遗传育种重点实验室，上海，201106;3.江西农业大学江西省动物生物技术重点开放实验室，南昌，330045;4.南京师范大学生命科学学院，南京，210097 |
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| 基金项目： | 上海市农业科学院中青年学术和技术带头人培养基金 |
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| 摘 要： | 利用密度梯度离心等方法从孵化51-56 h的石歧杂鸡胚血液中提取PGCs，用自制的玻璃注射针将PGCs注射到孵化2.5 d的H系受体鸡胚中制备种系嵌和体鸡；通过筛选AFLP引物建立起家禽嵌和体的AFLP检测方法；经检测20个发育的PGCs受体鸡胚中有８个种系嵌和体，嵌和率为40%。
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| 关 键 词： | 种系嵌和体 家禽原始生殖细胞 扩增片段长度多态性 |
| 文章编号： | 0253-9772(2005)03-0367-05 |
| 收稿时间： | 2004-06-09 |
| 修稿时间： | 2004年6月9日 |
Generation of Chicken Germ-line Chimeras by Transferring PGCs and Their Identification by AFLP |
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| HU Xiao-Fen,XIE Bei,YU Rui-song,HUANG Qi-zhong,ZHANG De-fu,HUANG Lu-sheng,LI Zhen.Generation of Chicken Germ-line Chimeras by Transferring PGCs and Their Identification by AFLP[J].Hereditas,2005,27(3):367-371. |
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| Authors: | HU Xiao-Fen XIE Bei YU Rui-song HUANG Qi-zhong ZHANG De-fu HUANG Lu-sheng LI Zhen |
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| Institution: | 1. Division of Animal Genetic Engineering, Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Shanghai 201106,China ;2.Animal Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences , Shanghai 201106,China;3.Jiangxi Provincial Key Laboratory for Animal Biotechnology,Jiangxi Agricultural University,Nanchang,330045,China;4.School of Life Science,Nanjing Normal University, Nanjing 210097,China |
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| Abstract: | PGCs (Primordial germ cells) were isolated from the blood of 51~56 h hatching Shiqiza chicken embryos by Ficoll density gradient centrifugation. The PGCs were injected into 2.5 d hatching embryos of H breed chicken to produce germ-line chimeras. AFLP checking method was established to identify chicken germline chimeras. Eight germ-line H-S chimera embryos were identified among 20 developing H breed embryos. |
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| Keywords: | PGCs germline chimeras AFLP |
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