| 一种改进的cDNA文库固相构建法 |
| |
| 引用本文： | 王义琴,李文彬,张利明,孙勇如.一种改进的cDNA文库固相构建法[J].遗传,2000,22(4):253-254. |
| |
| 作者姓名： | 王义琴 李文彬 张利明 孙勇如 |
| |
| 作者单位： | 中国科学院遗传研究所,北京 100101
Institute of Genetics,Chinese Academy of Sciences,Beijing 100101,China |
| |
| 摘 要： | 本文介绍一种新的cDNA文库固相构建法。文库构建过程中，cDNA的合成和修饰全部在固相介质――磁珠上完成,简便、迅速、文库质量高，能满足不同目的的cDNA文库构建，是一种值得借鉴和应用的好方法。
Abstract：A method for cDNA library construction was introduced.All enzymatic steps of library synthesis were performed on a solid support-magnetic beads.Therefore it combines fast speed and convenient with high quality library,making it ideally suited for most purposes.It is a good method and worth learning and utilization.
|
| 关 键 词： | 磁珠 cDNA library 天麻抗真菌蛋白 Key words 固相构建 cDNA文库 |
| 修稿时间： | 1999－09－14 |
An Improved Method for Solid-phase cDNA Library Construction |
| |
| WANG Yi-qin,LI Wen-bin,ZHANG Li-ming,SUN Yong-ru.An Improved Method for Solid-phase cDNA Library Construction[J].Hereditas,2000,22(4):253-254. |
| |
| Authors: | WANG Yi-qin LI Wen-bin ZHANG Li-ming SUN Yong-ru |
| |
| Abstract: | A method for cDNA library construction was introduced.All enzymatic steps of library synthesis were performed on a solid support magnetic beads.Therefore it combines fast speed and convenient with high quality library,making it ideally suited for most purposes.It is a good method and worth learning and utilization. |
| |
| Keywords: | cDNA library solid phase construction magnetic beads gastrodia antifungal protein |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《遗传》浏览原始摘要信息 |
| 点击此处可从《遗传》下载免费的PDF全文 |
