基于AFLP标记的中国西藏近缘野生大麦遗传多样性分析

选取7对引物组合, 构建了36份西藏近缘野生大麦和4份栽培大麦的AFLP指纹图谱, 共获得清晰可辨的条带227条, 其中多态性带194条, 占85.46％, 从DNA分子水平显示出所试材料遗传多样性较为丰富。计算得各样品间的遗传距离(欧氏距离)介于2.646~10.488之间, 应用离差平方和法对供试材料的AFLP数据结果进行聚类, 建立了40份大麦材料的AFLP树状图, 聚类结果将40份材料分为5类, 进一步揭示出供试材料间遗传背景的相似性和复杂性。结合Nei’s遗传一致性分析结果, 发现近缘六棱野生大麦较之近缘二棱野生与栽培大麦的亲缘关系更近, 支持栽培大麦是从野生二棱大麦起源, 而野生六棱大麦是进化过程中的过渡类型的大麦系统发生观点。

Genetic diversity of wild close relatives of barley in Tibet of China revealed by AFLP

Seven primer combinations were used for AFLP analysis. The fingerprints of 36 wild close relatives of bar-ley in Tibet of China and 4 cultivated varieties of barley had been obtained,bringing about 227 discernable bands. Among the bands 194 were polymorphic with the percentage of 85.46%,indicating that the materials in the test have abundant ge-netic diversity. The genetic distance(Euclidean Distance) of samples computed by SPSS is between 2.646 and 10.488. Ap-plying Ward's method to cluster the AFLP data results,an AFLP dendrogram of 40 barley materials was then established. All samples were clustered into 5 groups,further revealing the similarity,complicacy of the genetic background and the relationship of phylogenesis of the barley materials tested. Consulting the genetic identity it was shown that six-rowed wild close barley is closer to cultivated barley than two-rowed wild close barley. This result supported the viewpoint that the six-rowed wild close barley was the intermediate type in the evolutionary route from the tow-rowed wild close barley to the cultivated barley.