黑腹果蝇单胚培养技术的建立

过硬单胚培养十一项特殊的技术，运用此技术可以从果蝇单个原肠胚（同一基因型）中提取细胞进行体外培养，科观察到各种细胞的分化过程，从而能研究导致发生异常或致死突变的机制。通过反复摸索实验，我们在本实验室的条件下，建立了黑腹果蝇（D. melanogaster）单胚培养技术，并对野生型（Canton special, CS.）果蝇的单胚细胞进行体外培养，观察到了各种细胞，如肌肉细胞、神经细胞、脂肪细胞、以及成虫盘等的分化及其发生规律性，为今后研究突变奠定了基础。实验过程中，我们采用了载玻片法和培养皿法。两种方法各有特点及利弊，本文一并进行讨论。Abstract:We can culture the cells of single gastrula (the gene type of cells is same) in vitro,we now have cstablished the technique of single embryo culture in Drosophila.We have seen the differentiated state of myocytes, neurocytes,hemocyte cells,epithelical cells and imagical disk by this technique.

The Establishment of Single Embryo Culture Technique in Drosophila melanogaster

We can culture the cells of single gastrula(the gene type of cells is same) in vitro, we now have estublished the technique of single embryo culture in Drosophila. We have seen the differentiatde state of myocytes, neurocytes, hemocyte cells, fat cells, cpithelical cells and imagical disk by this technique.