| 豚鼠精子在发生及顶体反应过程中细胞内Ca~(2+)的定位研究 |
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| 引用本文： | 杨增明,陈大元.豚鼠精子在发生及顶体反应过程中细胞内Ca~(2+)的定位研究[J].动物学报,1992(4). |
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| 作者姓名： | 杨增明 陈大元 |
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| 作者单位： | 中国科学院动物研究所生殖生物学开放研究实验室，中国科学院动物研究所生殖生物学开放研究实验室 北京 100080，北京 100080 |
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| 摘 要： | 实验利用焦锑酸钾法对豚鼠精子在发生及顶体反应过程中的Ca~(2+)定位作了较详细的研究。在精母细胞及精子细胞上都有Ca~(2+)分布,但睾丸中的精子上则无Ca~(2+)。成熟精子中Ca~(2+)主要定位于顶体帽的整个腹面及背面的两个特定区域。发生顶体反应的精子上Ca~(2+)则位于顶体外膜上或囊泡内,已发生顶体反应的精子中Ca~(2+)则位于顶体内膜上。
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| 关 键 词： | 豚鼠 精子 Ca~(2+) 顶体反应 精子发生 |
LOCALIZATION OF INTRACELLULAR Ca~(2+) DURING SPERMATOGENE- SIS AND ACROSOME REACTION IN GUINEA PIG SPERMATOZOA |
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| YANG ZENG-MING CHEN DA-YUAN.LOCALIZATION OF INTRACELLULAR Ca~(2+) DURING SPERMATOGENE- SIS AND ACROSOME REACTION IN GUINEA PIG SPERMATOZOA[J].Acta Zoologica Sinica,1992(4). |
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| Authors: | YANG ZENG-MING CHEN DA-YUAN |
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| Abstract: | The localization of intracellular calcium of Guinea pig spermatozoa during the sperma-togenesis and acrosome reaction is studied using potassium pyroantimonate method. Calcium distributes sparsely in spermatocytes and spermatides, but is not found in testicular spermatozoa. In matured spermatozoa, calcium is mainly localized at whole ventral side of acrosome cap and two special regions of dorsal side of acrosome cap. Calcium is localized on the outer acrosome membrane or in the vesicles of the spermatozoa undergoing the acrosome reaction, but on the inner acrosome membrane of he acrosome-reacted spermatozoa. |
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| Keywords: | Guinea pig Spermatozoa Calcium Acrosome reaction Spermatogenesis |
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