Cryopreservation of sweet potato (Ipomoea batatas [L.] Lam.) shoot tips by vitrification |
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| Authors: | Leigh E Towill Robert L Jarret |
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| Institution: | (1) USDA/ARS, National Seed Storage Laboratory, Colorado State University, 80523 Fort Collins, CO, USA;(2) USDA/ARS, Southern Regional Plant Introduction Station, 1109 Experiment Street, 30223 Griffin, GA, USA |
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| Abstract: | Summary Vitrification is a technically simple method for cryopreserving plant germplasm, requiring only the application of suitable cryoprotectants and rapid cooling rates. Sweetpotato (Ipomoea batatas L.] Lam.) shoot tips obtained from in vitro plants survived liquid nitrogen (–196°C) exposure following a vitrification-inducing pretreatment. Shoot tips were treated in a stepwise manner with a vitrification solution containing 30% glycerol, 15% ethylene glycol and 15% dimethylsulfoxide in growth medium. Incubation of shoot tips for 1 to 2 h in low concentrations of the vitrification solution enhanced survival. Most surviving shoot tips developed callus, and a variable percentage subsequently formed shoots. Survival was not achieved using two-step cooling procedures. The percentage of shoot tips surviving vitrification and those subsequently forming a shoot varied widely among replications.Abbreviations BA
N6-benzyladenine
- IBA
indole-3-butyric acid
- EG
ethylene glycol
- DMSO
dimethylsulfoxide
- MS
Murashige and Skoog (1962) minerals and vitamins
- LN
liquid nitrogen
- PI
plant introduction |
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| Keywords: | clones cryoprotectants germplasm preservation shoot tip culture |
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