| 化学合成虎纹捕鸟蛛毒素-I基因的克隆和表达 |
| |
| 引用本文： | 李敏,李路怡,梁宋平.化学合成虎纹捕鸟蛛毒素-I基因的克隆和表达[J].生命科学研究,1998(3). |
| |
| 作者姓名： | 李敏 李路怡 梁宋平 |
| |
| 作者单位： | 湖南师范大学生物学系 |
| |
| 基金项目： | 国家自然科学基金,国家“863”课题 |
| |
| 摘 要： | 本文报道了全化学合成虎纹捕鸟蛛毒素－Ⅰ基因在大肠杆菌中的表达，表达产物为Ｎ－端是谷胱甘肽硫转移酶的融合蛋白．经ＧＳＨ－Ｓｅｐｈａｒｏｓｅ４Ｂ亲和层析纯化，凝血酶酶解融合蛋白，得到重组ＨＷＴＸ－Ⅰ（ｒＨＷＴＸ－Ⅰ）．质谱和氨基酸顺序分析均表明ｒＨＷＴＸ－Ⅰ系正确表达产物．还原复性的ｒＨＷＴＸ－Ⅰ表现出与天然ＨＷＴＸ－Ⅰ生物学活性的一致性．
|
| 关 键 词： | 蜘蛛，虎纹捕鸟蛛毒素－Ⅰ基因，化学合成，原核表达 |
Cloning and Expression of Chemical Synthetic HWTX I Gene |
| |
| LI,Min,LI,Luyi,LIANG,Songping.Cloning and Expression of Chemical Synthetic HWTX I Gene[J].Life Science Research,1998(3). |
| |
| Authors: | LI Min LI Luyi LIANG Songping |
| |
| Abstract: | The chemical synthetic HWTX I gene was expressed in E.coli as a hybrid protein fused with glutathione S transferase(GST) at N terminal. The fusion protein was purified by GSH Sepharose 4B affinity column chromatography,and was cleaved by thrombin to obtain the recombinant HWTX I (rHWTX I).Mass spectrometry and amino acid sequence analysis proved that rHWTX I was expressed correctly.Renatured rHWTX I showed the same biological property with that of the native HWTX I. |
| |
| Keywords: | spider Huwentoxin I gene chemical synthesis prokaryotic expression |
| 本文献已被 CNKI 等数据库收录！ |
|
