Biodegradation of Ethametsulfuron-Methyl by <Emphasis Type="Italic">Pseudomonas</Emphasis> sp. SW4 Isolated from Contaminated Soil |
| |
Authors: | Gu Li-feng Jiang Jian-dong Li Xiao-hui Shinawar Waseem Ali Li Shun-peng |
| |
Institution: | (1) Key Laboratory of Microbiological Engineering of Agricultural Environment, Nanjing Agricultural University, Nanjing, 210095, Peoples Republic of China |
| |
Abstract: | A soil bacterium SW4, capable of degrading the sulfonylurea herbicide ethametsulfuron-methyl (ESM), was isolated from the
bottom soil of a herbicide factory. Based on physiological characteristics, biochemical tests and phylogenetic analysis of
the 16S rRNA gene sequence, the strain was identified as a Pseudomonas sp. The total degradation of ESM in the medium containing glucose was up to 84.6% after 6 days of inoculation with SW4 strain.
The inoculation of strain SW4 to soil treated with ESM resulted in a higher degradation rate than in noninoculated soil regardless
of the soil sterilized or nonsterilized. Five metabolites of ESM degradation were analyzed by liquid chromatography/mass spectrometry.
Based on the identified products, strain SW4 seemed to degrade ESM after two separate and different pathways: one leads to
the cleavage of the sulfonylurea bridge, whereas the other to the dealkylation and opening of the triazine ring of ESM. |
| |
Keywords: | Ethametsulfuron-methyl Biodegradation Pseudomonas sp SW4 |
本文献已被 PubMed SpringerLink 等数据库收录! |
|