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A soil bacterium SW4, capable of degrading the sulfonylurea herbicide ethametsulfuron-methyl (ESM), was isolated from the
bottom soil of a herbicide factory. Based on physiological characteristics, biochemical tests and phylogenetic analysis of
the 16S rRNA gene sequence, the strain was identified as a Pseudomonas sp. The total degradation of ESM in the medium containing glucose was up to 84.6% after 6 days of inoculation with SW4 strain.
The inoculation of strain SW4 to soil treated with ESM resulted in a higher degradation rate than in noninoculated soil regardless
of the soil sterilized or nonsterilized. Five metabolites of ESM degradation were analyzed by liquid chromatography/mass spectrometry.
Based on the identified products, strain SW4 seemed to degrade ESM after two separate and different pathways: one leads to
the cleavage of the sulfonylurea bridge, whereas the other to the dealkylation and opening of the triazine ring of ESM. 相似文献
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