Thiamin biosynthesis in prokaryotes |
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| Authors: | T P Begley Diana M Downs Steven E Ealick Fred W McLafferty Adolphus P G M Van Loon Sean Taylor Nino Campobasso Hsiu-Ju Chiu Cynthia Kinsland Jason J Reddick Jun Xi |
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| Institution: | (1) Department of Chemistry and Chemical Biology, 120 Baker Laboratory, Cornell University, Ithaca, NY 14853, USA e-mail: tpb2@cornell.edu Tel. +1-607-255-7133; Fax +1-607-255-4137, US;(2) Department of Bacteriology, University of Wisconsin-Madison, 1550 Linden Drive, Madison, WI 53706, USA, US;(3) Biotechnology Section, F. Hoffmann-La Roche Ltd., Grenzacherstrasse 124, CH-4070 Basel, Switzerland, CH |
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| Abstract: | Twelve genes involved in thiamin biosynthesis in prokaryotes have been identified and overexpressed. Of these, six are required
for the thiazole biosynthesis (thiFSGH, thiI, and dxs), one is involved in the pyrimidine biosynthesis (thiC), one is required for the linking of the thiazole and the pyrimidine (thiE), and four are kinase genes (thiD, thiM, thiL, and pdxK). The specific reactions catalyzed by ThiEF, Dxs, ThiDM, ThiL, and PdxK have been reconstituted in vitro and ThiS thiocarboxylate
has been identified as the sulfur source. The X-ray structures of thiamin phosphate synthase and 5-hydroxyethyl-4-methylthiazole
kinase have been completed. The genes coding for the thiamin transport system (thiBPQ) have also been identified. Remaining problems include the cloning and characterization of thiK (thiamin kinase) and the gene(s) involved in the regulation of thiamin biosynthesis. The specific reactions catalyzed by
ThiC (pyrimidine formation), and ThiGH and ThiI (thiazole formation) have not yet been identified.
Received: 23 August 1998 / Accepted: 16 January 1999 |
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| Keywords: | Thiamin Biosynthesis Prokaryotes Transport Kinase Mechanism Structure |
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