大口黑鲈生长相关标记的聚合及其效果分析

为了解与大口黑鲈(Micropterus salmoides)生长性状相关分子标记优势基因型的聚合效果, 研究选择先前已获得的13个与生长性状相关的分子标记, 位于PCK1、HSBP1、FOXO3b、MYH、HSC70-1、CTSB、HBP、POU1F1、PACAP、IGF-I、ghrelin、ApoproteinA1和MSTN基因上。在40尾亲本群体中对各标记的基因型进行检测, 选择可聚合优势基因型最多的2对亲本分别构建家系。在9月龄时, 从2个家系子代中分别采集305尾和266尾个体进行生长性状测量和各标记的基因型分析。家系1子代个体中含生长相关优势基因型的数量为0—6个, 对应的个体数依次为8、26、75、74、76、35和11, 对应的平均体质量依次为185.03、196.46、198.73、212.59、222.66、235.54和261.27 g。家系2子代个体中含生长相关优势基因型的数量为1—6个, 对应的平均体质量依次为184.43、213.17、243.77、249.98、252.11和266.00 g。个体中生长相关优势基因型聚合数量多少与生长性状呈正相关, 提示通过对生长相关优势基因型进行聚合可获得生长性状优良的大口黑鲈, 为大口黑鲈分子标记辅助育种的应用提供科学依据。

ANALYSIS OF THE PYRAMIDING EFFECT OF GROWTH-RELATED MOLECULAR MARKERS IN LARGEMOUTH BASS (MICROPTERUS SALMOIDES)

Growth traits are controlled by multiple genes to cause minor phenotypic effect. To understand the pyra-miding effect of advantage genotypes of growth-related molecular markers in largemouth bass (Micropterus salmoides), thirteen molecular markers related to growth traits were selected obtained on previous studies, which were loca-ted in PCK1, HSBP1, FOXO3b, MYH, HSC70-1, CTSB, HBP, POU1F1, PACAP, IGF-I, ghrelin, ApoproteinA and MSTN. The genotype of each marker in forty largemouth bass parents was analyzed and 2 families were constructed basing on pyramiding advantage genotypes. 305 and 266 individuals were sampled randomly from second filial generation of two families at nine month old, respectively. The results showed that the number of advantage genotypes was various in all offspring of family 1 with the number of individuals of each group 8, 26, 75, 74, 76, 35 and 11, respecti-vely. The average weight of advantage genotypes group was 185.03, 196.46, 198.73, 212.59, 222.66, 235.54 and 261.27 g, respectively. In the offspring of family 2, the number of advantage genotype ranged from one to six with the average weight of 184.43, 213.17, 243.77, 249.98, 252.11 and 266.00 g, respectively. Correlation analysis showed that the pyramiding number of advantage genotype was correlated with growth traits. Our results present evidence to improve the growth trait of largemouth bass by utilizing the limited advantage genotypes. This study provided scientific basis for the application of molecular marker assisted breeding in largemouth bass.