| 微生物酶解法产生阿拉伯糖——菌种筛选与初步鉴定 |
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| 引用本文： | 周俊,徐畅,浦佳,彭哲初,邓亚卉,徐洁,贾香清,郑珩.微生物酶解法产生阿拉伯糖——菌种筛选与初步鉴定[J].氨基酸和生物资源,2010,32(4):73-76. |
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| 作者姓名： | 周俊 徐畅 浦佳 彭哲初 邓亚卉 徐洁 贾香清 郑珩 |
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| 作者单位： | 中国药科大学生命科学与技术学院,南京,210009;中国药科大学生命科学与技术学院,南京210009;中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室,南京210008 |
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| 基金项目： | 土壤与农业可持续发展国家重点实验室开放基金;项目号Y052010040，国家大学生创新实验计划;项目号:J0630858 |
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| 摘 要： | 使用半纤维素平板法从土壤分离菌中筛选到产半纤维素酶活较高的真菌。将这些菌株在含玉米芯半纤维素的培养基中发酵,鉴定发酵液中还原糖产量,从中筛选出3株产还原糖较多的菌。将其发酵产物经柱前衍生,HPLC检测,发现其中DHC菌株的发酵产物以木糖和阿拉伯糖为主,而培养基中的葡萄糖大部分被利用,可用于发酵法联产阿拉伯糖和木糖。对DHC菌株通过菌落形态、孢子形态以及ITS区基因序列测序分析,初步确定该菌为亮白曲霉(Aspergillus candidus)。对于亮白曲霉产生的半纤维素酶国内外尚较少研究报导,可为开发利用新的微生物资源奠定基础。
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| 关 键 词： | 半纤维素 菌种筛选 ITS序列分析 亮白曲霉 |
Microbial Production of Arabinose:(I)Screening and Identification of Strains |
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| ZHOU Jun,XU Chang,PU Jia,PENG Zhe-chu,DENG Ya-hui,XU Jie,JIA Xiang-qing,ZHENG Heng.Microbial Production of Arabinose:(I)Screening and Identification of Strains[J].Amino Acids & Biotic Resources,2010,32(4):73-76. |
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| Authors: | ZHOU Jun XU Chang PU Jia PENG Zhe-chu DENG Ya-hui XU Jie JIA Xiang-qing ZHENG Heng |
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| Institution: | ZHOU Jun1,XU Chang1,PU Jia1,PENG Zhe-chu1,DENG Ya-hui1,XU Jie1,JIA Xiang-qing1,ZHENG Heng1,2,(1China Pharmaceutical University,Nanjing 210009,2State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Sciences,Nanjing 210008,China) |
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| Abstract: | Hemicellulase producing fungi have been isolated from soil by hemicellulose plate screening method.These fungi were incubated in hemicellulose medium,and the resulting reductive sugars were detected by DNS method.By this method,3 strains were selected with high hemicellulase activity.Furthermore,to verify whether these strains could produce arabinose,its products were detected by HPLC after PMP derivation.In the fermentation of DHC strain,the produced sugars were mainly xylose and arabinose,while glucose co... |
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| Keywords: | hemicellulose screening of strain ITS analysis Aspergillus candidus |
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