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藿香枝叶多糖的提取工艺及其清除羟基自由基作用研究
引用本文:刘存芳.藿香枝叶多糖的提取工艺及其清除羟基自由基作用研究[J].氨基酸和生物资源,2012,34(3):1-3.
作者姓名:刘存芳
作者单位:陕西理工学院,化学与环境科学学院,陕西,汉中,723000
基金项目:陕西省科技厅社会发展科技攻关项目(2012K19-03-03);陕西省教育厅专项科研计划项目(2010JK471)
摘    要:采用苯酚-硫酸,并以超声法辅助提取野生藿香枝叶中的多糖,设计L9(34)正交试验考察料液比、pH和超声提取时间对藿香枝叶中多糖提取的影响,并对藿香多糖进行清除.OH试验。结果表明,藿香多糖的最佳提取工艺为料液比1∶10,超声提取30min,pH值为6,可使藿香多糖的提取率高达7.50%。藿香多糖对羟基自由基有显著地清除效果,表明其有抗氧化作用。

关 键 词:藿香枝叶  多糖  正交试验  抗氧化作用

Study on Extraction and Hydroxyl Radical-Scavenging of Polysaccharide from Branches and Leaves of Agastache rugosa
LIU Cunfang.Study on Extraction and Hydroxyl Radical-Scavenging of Polysaccharide from Branches and Leaves of Agastache rugosa[J].Amino Acids & Biotic Resources,2012,34(3):1-3.
Authors:LIU Cunfang
Institution:LIU Cunfang(College of Chemistry and Environmental Science,Shaanxi University of Technology,Hanzhong 723000,Shaanxi,China)
Abstract:Agastache rugosa polysaccharide was extracted from the branches and leaves of Agastache rugosa by ultrasonic extraction method,and L9(34) orthogonal experiment based on material-liquid ratio,ultrasonic time and pH was tested to optimize the extraction technology.Agastache rugosa polysaccharide was also used to remove hydroxyl radical.The results showed that the best extracting technology was material-liquid ratio of 1∶10,ultrasonic time for 30 min,and pH 6.The extraction rate of polysaccharide was reached up to 7.50%.Agastache rugosa polysaccharide had antioxidant activity,and had significant scavenging effect for hydroxyl radical.
Keywords:Agastache rugosa branches and leaves  polysaccharide  orthogonal experiment  anti-oxidation activity
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