| 葛根素注射液中葛根素含量的HPLC测定 |
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| 引用本文： | 鄢贵龙,夏敏.葛根素注射液中葛根素含量的HPLC测定[J].氨基酸和生物资源,2005,27(3):80-82. |
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| 作者姓名： | 鄢贵龙 夏敏 |
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| 作者单位： | 1. 淮阴师范学院生物系,江苏,淮安,223300;2. 淮阴师范学院分析测试中心,江苏,淮安,223300 |
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| 摘 要： | 建立了一种简便、准确、可靠的葛根素测定方法。采用SymmetryC18(3.9mm×150mm,5μm)柱,流动相采用甲醇-水(体积比为3525),流速为1.00ml·min-1,检测波长为250nm。进样量在0.0368μg～0.589μg范围内线性关系良好,(r=0.9994)。该方法简便快速,准确可靠,可用于葛根素注射液的含量测定。同时,测定结果表明两种产品在含量上有差别,因此,保证和进一步提高葛根素注射液质量,并且规范质量标准势在必行。
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| 关 键 词： | 葛根素 葛根素注射液 含量测定 HPLC |
| 文章编号： | 1006-8376(2005)03-0080-03 |
| 收稿时间： | 05 17 2005 12:00AM |
| 修稿时间： | 2005年5月17日 |
Determination of Puerarin Coutent in Puerarin injection by HPLC |
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| YAN Gui-long,XIA Min.Determination of Puerarin Coutent in Puerarin injection by HPLC[J].Amino Acids & Biotic Resources,2005,27(3):80-82. |
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| Authors: | YAN Gui-long XIA Min |
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| Institution: | , 2.Instrumental Analysis Center, huaiyin teachers college, Huaian 223300,China) |
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| Abstract: | To establish a method for determination of puerarin, puerarin content of the products was determined by HPLC in Symmetry C18( 3.9mm×150mm,5μm) using a mobile phase of MeOH:H2O=35:65 and a UV detector of 250nm. A standard curve was abtained with a liner range of 0.0368μg～0.589μg(r=0.9994). The method was simple and accurate and could be used for determination of purerarin in puerarin injection. |
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| Keywords: | HPLC |
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