| 假单胞菌N-13中丝氨酸羟甲基转移酶的酶学性质研究 |
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| 引用本文： | 张超,栾兴社,张春明,薛维纳.假单胞菌N-13中丝氨酸羟甲基转移酶的酶学性质研究[J].氨基酸和生物资源,2011,33(1):39-42. |
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| 作者姓名： | 张超 栾兴社 张春明 薛维纳 |
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| 作者单位： | 山东建筑大学,市政与环境工程学院,济南,250101;山东建筑大学,市政与环境工程学院,济南,250101;山东建筑大学,市政与环境工程学院,济南,250101;山东建筑大学,市政与环境工程学院,济南,250101 |
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| 摘 要： | 从产L-丝氨酸菌株假单胞菌N-13中纯化了丝氨酸羟甲基转移酶,并对其性质进行了研究.结果表明,丝氨酸羟甲基转移酶酶活力在pH=7.0～9.0间稳定,最适宜pH=8.0;酶的最适温度为35℃,在30～40℃水浴30 min酶活力未见明显下降.磷酸吡哆醛的最适添加浓度为25 μmol·L-1.研究了不同金属离子对酶活力的影...
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| 关 键 词： | 假单胞菌 L-丝氨酸 丝氨酸羟甲基转移酶 酶学性质 |
Study on the Properties of SHMT from Pseudomonas N-13 |
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| ZHANG Chao,LUAN Xing-she,ZHANG Chun-ming,XUE Wei-na.Study on the Properties of SHMT from Pseudomonas N-13[J].Amino Acids & Biotic Resources,2011,33(1):39-42. |
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| Authors: | ZHANG Chao LUAN Xing-she ZHANG Chun-ming XUE Wei-na |
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| Institution: | ZHANG Chao,LUAN Xing-she,ZHANG Chun-ming,XUE Wei-na (School of Municipal and Environmental Engineering,Shandong Jianzhu University,Jinan 250101,China) |
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| Abstract: | SHMT of L-serine-producing strain of Pseudomonas N-13 was purified and characterized.The enzyme was stable between pH 7.0 and 9.0 with a optimum pH=8.0.The optimum temperature was 35℃,and no significant activity loss was observed when the enzyme was incubated at 30～40℃ for 30min.The optimal concentration of PLP was 25μmol·L-1.The effect of different metal ions on the activity of the enzyme were also investigated.Some metal ions,including Cu 2+ and Co 2+,could increase the enzyme activities by 22% and 10%,respectively,while some other metal ions,including Mn 2+,Mg 2+ and Zn 2+,strongly inhibited the enzyme activity.The enzyme activity was not affected by Ca 2+.The ability of accumulating L-serine in the mutants was relevant with SHMT activity,higher SHMT activity,more yield of L-serine. |
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| Keywords: | Pseudomonas L-serine SHMT enzymetic property |
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