| 韩芝液体深层发酵培养基的优化研究 |
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| 引用本文： | 武忠伟,许桂芳,赵现方,窦艳萍.韩芝液体深层发酵培养基的优化研究[J].氨基酸和生物资源,2007,29(4):29-32. |
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| 作者姓名： | 武忠伟 许桂芳 赵现方 窦艳萍 |
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| 作者单位： | 河南科技学院,生物工程系,河南,新乡,453003 |
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| 基金项目： | 河南科技学院重点科研项目资助基金(5048) |
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| 摘 要： | 固体培养基上比较了韩芝与树舌的菌丝生长速度,二者平均分别达到0.77 cm.d-1和0.67 cm.d-1;液体培养试验了不同碳氮比、培养基组成对韩芝菌丝生长的影响,得出了菌丝生长较适宜的碳氮比范围在18~24∶1,最适宜的碳氮比在22∶1;对比试验、正交试验得出韩芝液体培养最佳的培养基配方为(g.L-1):葡萄糖10.0、玉米粉20.0、大豆粉20.0、酵母粉5.0;在此条件下,菌丝的平均产量可达23.12 g.L-1。
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| 关 键 词： | 韩芝 深层发酵 培养基 优化 |
| 文章编号： | 1006-8376（2007）04-0029-04 |
| 收稿时间： | 2007-06-01 |
| 修稿时间： | 2007年6月1日 |
Study On the Liquid Fermentation Medium Optimization of Ganoderma lucidum HG |
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| WU Zhong-wei,XU Gui-fang,ZHAO Xian-fang,DOU Yan-ping.Study On the Liquid Fermentation Medium Optimization of Ganoderma lucidum HG[J].Amino Acids & Biotic Resources,2007,29(4):29-32. |
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| Authors: | WU Zhong-wei XU Gui-fang ZHAO Xian-fang DOU Yan-ping |
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| Abstract: | The growth speed of Ganoderma lucidum HG and Ganoderma applanatumuan was compared,0.770cm·d-1 and 0.67 cm·d-1 respectively.The effect of the ratio of C/N and the combination of medium were tested in liquid culture.The results showed that the better C/N was 20-24:1,the perfect was 22:1.The optimal fermentation medium were investigated by the contrast experiment and orthogonal devices.The optimum ingredient combination(g/L):glucose 10.0,corn powder 20.0,soya flour 20.0,yeast extract 5.0.In the conditions,the maximal biomass was 23.12g·L/-1. |
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| Keywords: | Ganoderma lucidum HG fermentation medium optimization |
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