| 北细辛内生真菌的分离鉴定及代谢产物的生物活性 |
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| 引用本文： | 包丽霞,殷瑜,杨天,杨和行,钱秀萍.北细辛内生真菌的分离鉴定及代谢产物的生物活性[J].微生物学杂志,2010,30(5):1-6. |
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| 作者姓名： | 包丽霞 殷瑜 杨天 杨和行 钱秀萍 |
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| 作者单位： | 1. 上海交通大学,药学院,上海,200240
2. 上海来益生物药物研发中心,上海,201203 |
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| 基金项目： | 国家重大新药创制专项 |
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| 摘 要： | 采用表面消毒法分别从3种北细辛中分离获得10株形态特征不同的优势内生真菌,经形态学和18S rDNA ITS分子分类学分析鉴定为小丛壳属(Glomerella sp.)、叶点霉属(Phyllosticta sp.)、柄孢壳菌属(Po-dospora sp.)、刺盘孢属(Colletotrichum sp.)和镰孢属(Fusarium sp.)。对北细辛优势内生真菌的发酵产物进行体外抗肿瘤和抗菌活性检测,结果表明:除菌株E3、E4和E10外,其余菌株均有不同程度的抗肿瘤和抗菌活性;镰孢属(Fusarium sp.)菌株E9对A549、MDA-MB-231和PANC-1肿瘤细胞抑制率达75%以上;小丛壳属(Glomerella sp.)菌株E1和叶点霉属(Phyllosticta sp.)菌株E2靶向FabI的抗菌活性较强,抑制率达59%。
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| 关 键 词： | 北细辛 内生真菌 分离 抗肿瘤活性 抗菌活性 |
Isolation,Identification and Metabolite Bioactivity of Endophytic Fungi from Asarum heterotropoides var. mandshuricum. |
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| BAO Li-xi,YIN Yu,YANG Tian,YANG He-xing,QIAN Xiu-ping.Isolation,Identification and Metabolite Bioactivity of Endophytic Fungi from Asarum heterotropoides var. mandshuricum.[J].Journal of Microbiology,2010,30(5):1-6. |
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| Authors: | BAO Li-xi YIN Yu YANG Tian YANG He-xing QIAN Xiu-ping |
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| Institution: | BAO Li-xia1,YIN Yu2,YANG Tian2,YANG He-xing1,QIAN Xiu-ping1(1.Sch.of Pharm.,Shanghai Jiaotong Uni.,Shanghai 200240,2.Shanghai Health Creation Ctr.for Biopharm.R & D,Shanghai 201203) |
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| Abstract: | 10 dominant endophytic fungal strains of different characteristics morphologically were isolated from three kinds of Asarum heterotropoides by the surface-sterilized method,and they were identified as Glomerella sp.,Phyllosticta sp.,Podospora sp.,Colletotrichum sp.and Fusarium sp.by morphology,18S rDNA internal transcribed spacer(ITS) molecular taxological analyses.The antitumor and antibacterial activities of their metabolites of these predominant endophytic fungi were detected in vitro.The results indicat... |
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| Keywords: | Asarum heterotropoides endophytic fungi isolation antitumor activities antibacterial activities |
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